Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all NIF1 Antibodies »

Product Summary for NIF1 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 300 and 350 of human NRC-Interacting Factor 1 using the numbering given in entry NP_071378.1 (GeneID 63925).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NIF1 Antibody

Immunohistochemistry: NIF1 Antibody [NB100-2578]

Immunohistochemistry: NIF1 Antibody [NB100-2578]

Immunohistochemistry: NIF1 Antibody [NB100-2578] - Sample: FFPE section of human thyroid carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-NIF1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunoprecipitation: NIF1 Antibody [NB100-2578]

Immunoprecipitation: NIF1 Antibody [NB100-2578]

Immunoprecipitation: NIF1 Antibody [NB100-2578] - Detection of Human NIF1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NIF1 antibody NB100-2578 used IP at 3 mcg/mg lysate. NIF1 was also immunoprecipitated by rabbit anti-NIF1 antibodies NB100-2579 and another NIF1 Ab. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for NIF1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
Not recommended for Western Blot. Use NB 100-2579
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NIF1

NIF1 enhances transcriptional activation by ligand-bound nuclear hormone receptors. However, it does this not by direct interaction with the receptor, but by direct interaction with the nuclear hormone receptor transcriptional coactivator NRC. This protein may function by altering local chromatin structure.

Alternate Names

DKFZp586G1122, Hzf, HZFzinc finger protein 385, RZFHematopoietic zinc finger protein, ZFP385, zinc finger protein 385A, ZNF385Retinal zinc finger protein

Entrez Gene IDs

63925 (Human)

Gene Symbol

ZNF335

UniProt

NP_071378.1 (Human)

Additional NIF1 Products

Product Documents for NIF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NIF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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