NIF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2579

Novus Biologicals, part of Bio-Techne
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NB100-2579
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all NIF1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1300 and the C-terminus (residue 1342) of human NRC-Interacting Factor 1 using the numbering given in entry NP_071378.1 (GeneID 63925).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NIF1 Antibody

Western Blot: NIF1 Antibody [NB100-2579]

Western Blot: NIF1 Antibody [NB100-2579]

Western Blot: NIF1 Antibody [NB100-2579] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. NB100-2579 used for WB at 0.04 ug/ml (A) and at 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). NIF1 was also immunoprecipitated by rabbit anti-NIF1 antibody BL2737 (NB100-2579) and BL2738. Detection was by chemiluminescence, with exposure times of 3 minutes (A) and 30 seconds (B).
Immunohistochemistry-Paraffin: NIF1 Antibody [NB100-2579]

Immunohistochemistry-Paraffin: NIF1 Antibody [NB100-2579]

Immunohistochemistry-Paraffin: NIF1 Antibody [NB100-2579] - Section of human Hodgkin's lymphoma. Antibody: Affinity purified rabbit anti- NIF1 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for NIF1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100 -1:500

Immunohistochemistry-Paraffin

1:100- 1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NIF1

NIF1 enhances transcriptional activation by ligand-bound nuclear hormone receptors. However, it does this not by direct interaction with the receptor, but by direct interaction with the nuclear hormone receptor transcriptional coactivator NRC. This protein may function by altering local chromatin structure.

Alternate Names

DKFZp586G1122, Hzf, HZFzinc finger protein 385, RZFHematopoietic zinc finger protein, ZFP385, zinc finger protein 385A, ZNF385Retinal zinc finger protein

Entrez Gene IDs

63925 (Human); 329559 (Mouse)

Gene Symbol

ZNF335

UniProt

NP_071378.1 (Human)

Additional NIF1 Products

Product Documents for NIF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NIF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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