NIFK Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56660

Novus Biologicals, part of Bio-Techne
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NB100-56660
NB100-56660SS
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all NIFK Antibodies »

Product Specifications

Immunogen

This antibody was developed against synthetic peptides corresponding to amino acids 144-160 and 276-293 of human NIFK. Amino acids 144-160 share a 40% homology with mouse NIFK.

Reactivity Notes

Predicted to cross react with Mouse.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NIFK Antibody

Western Blot: NIFK Antibody [NB100-56660]

Western Blot: NIFK Antibody [NB100-56660]

Western Blot: NIFK Antibody [NB100-56660] - Analysis of NIFK in 30 ugs of HeLa cell lysate using NB100-56660 at 1:1000.
Immunohistochemistry: NIFK Antibody [NB100-56660]

Immunohistochemistry: NIFK Antibody [NB100-56660]

Immunohistochemistry: NIFK Antibody [NB100-56660] - Staining in adipocytes of human carcinomatus breast tissue using NB100-56660 at 1:500 dilution.

Applications for NIFK Antibody

Application
Recommended Usage

Western Blot

1:500-1:1000
Application Notes
In HeLa, a 34 kDa band is observed. IHC: Formalin-fixed paraffin-embedded breast carcinoma.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.2% gelatin

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NIFK

NIFK is a nucleolar protein that interacts with Ki67, a protein strongly associated with, and a specific marker of, cell proliferation. The marsupial counterpart of human NIFK was recently identified and suggests that NIFK may be involved in the organization of higher order chromatin structure. NIFK was found to interact with the forkhead associated domain of Ki67 in mitotic cells and the interaction is thought to be phosphorylation-dependent.

Alternate Names

hNIFK, MKI67 (FHA domain) interacting nucleolar phosphoprotein, MKI67 FHA domain-interacting nucleolar phosphoprotein, NIFKNOPP34, Nopp34, Nucleolar phosphoprotein Nopp34, Nucleolar protein interacting with the FHA domain of pKI-67

Entrez Gene IDs

84365 (Human)

Gene Symbol

NIFK

UniProt

Q9BYG3 (Human)

Additional NIFK Products

Product Documents for NIFK Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NIFK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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