Ninein Antibody (PSH03-23)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32651
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH03-23
Concentration
1 mg/ml
Product Summary for Ninein Antibody (PSH03-23)
Immunogen
Recombinant protein within human Ninein aa 1-500 / 2,090. (Uniprot: Q8N4C6)
Localization
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
243 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Ninein Antibody (PSH03-23)
Western Blot: Ninein Antibody (PSH03-23) [NBP3-32651]
Western Blot: Ninein Antibody (PSH03-23) [NBP3-32651] - Western blot analysis of Ninein on different lysates with Rabbit anti-Ninein antibody (NBP3-32651) at 1/2,000 dilution.Lane 1: HeLa cell lysate
Lane 2: A549 cell lysate
Lane 3: HT-29 cell lysate
Lane 4: MEF cell lysate
Lane 5: Human lung tissue lysate
Lane 6: Mouse lung tissue lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 243 kDa
Observed band size: 270 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32651) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for Ninein Antibody (PSH03-23)
Application
Recommended Usage
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Ninein
Alternate Names
Glycogen synthase kinase 3 beta-interacting protein, GSK3B-interacting protein, hNinein, KIAA1565, ninein, ninein (GSK3B interacting protein), ninein centrosomal protein
Gene Symbol
NIN
Additional Ninein Products
Product Documents for Ninein Antibody (PSH03-23)
Product Specific Notices for Ninein Antibody (PSH03-23)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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