NIR2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1417

Novus Biologicals, part of Bio-Techne
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NB100-1417
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Key Product Details

Species Reactivity

Validated:

Mouse

Predicted:

Bovine (100%), Canine (100%), Human (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all NIR2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KARSISLKLDSEE corresponding to C-Terminus according to NP_004901.2, NP_001124320.1.

Localization

Cytoplasmic

Specificity

This antibody is expected to recognise both reported isoforms.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for NIR2 Antibody

Immunocytochemistry/ Immunofluorescence: NIR2 Antibody [NB100-1417]

Immunocytochemistry/ Immunofluorescence: NIR2 Antibody [NB100-1417]

Immunocytochemistry/Immunofluorescence: NIR2 Antibody [NB100-1417] - Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and Endoplasmic Reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Flow Cytometry: NIR2 Antibody [NB100-1417]

Flow Cytometry: NIR2 Antibody [NB100-1417]

Flow Cytometry: NIR2 Antibody [NB100-1417] - Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for NIR2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Peptide ELISA

Detection limit 1:8000
Application Notes
WB: An anonymous customer has reported a band at approx. 160 kDa band in lysates of a mouse Macrophage cell line after 2ug/ml antibody staining (calculated MW of 135 kDa band according to NP_004901.1). ICC: An anonymous customer has reported staining of vesicular cytoplasm in the same cell line.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NIR2

NIR2 belongs to a family of proteins that share homology with the Drosophila retinal degeneration B (rdgB) protein. Nir2, a human homolog of Drosophila melanogaster retinal degeneration B protein, is essential for cytokinesis. A specific Thr residue in the Nir2 PI-transfer domain provides a regulatory site for targeting to lipid droplets. This may affect intracellular lipid trafficking and distribution & explain the dominant effect of the RdgB-T59E mutant on retinal degeneration.

Alternate Names

DRES9NIR-2, Drosophila retinal degeneration B homolog, FLJ44997, membrane-associated phosphatidylinositol transfer protein 1, NIR2PITPNM, phosphatidylinositol transfer protein, membrane-associated 1Pyk2 N-terminal domain-interacting receptor 2, PITPnm 1, Rd9, RDGB, RDGB1, RDGBA, RDGBA1, retinal degeneration B alpha 1

Entrez Gene IDs

9600 (Human); 18739 (Mouse)

Gene Symbol

PITPNM1

UniProt

NP_004901. (Human)

Additional NIR2 Products

Product Documents for NIR2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NIR2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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