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NIT2 Antibody (OTI2B9) [Janelia Fluor® 549]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72995JF549

Novus Biologicals, part of Bio-Techne
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NBP2-72995JF549
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Janelia Fluor 549

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI2B9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Full length recombinant protein of human NIT2 (NP_064587) produced in HEK293T cells.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for NIT2 Antibody (OTI2B9) [Janelia Fluor® 549]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: NIT2

NIT2 has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates byconverting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate andoxaloacetate, respectively. Overexpression decr

Alternate Names

EC 3.5.1.3, MGC111199, Nit protein 2, nitrilase family, member 2, Nitrilase homolog 2, omega-amidase NIT2

Gene Symbol

NIT2

Additional NIT2 Products

Product Documents for NIT2 Antibody (OTI2B9) [Janelia Fluor® 549]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NIT2 Antibody (OTI2B9) [Janelia Fluor® 549]

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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