NIT2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89486

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-89486
NBP1-89486-25ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NIT2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YLIGGSIPEEDAGKLYNTCAVFGPDGTLLAKYRKIHLFDIDVPGKITFQESKTLSPGDSFSTFDTPYCRVGLGICYDMRFAELAQIYAQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NIT2 Antibody

Western Blot: NIT2 Antibody [NBP1-89486]

Western Blot: NIT2 Antibody [NBP1-89486]

Western Blot: NIT2 Antibody [NBP1-89486] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: NIT2 Antibody [NBP1-89486]

Immunocytochemistry/ Immunofluorescence: NIT2 Antibody [NBP1-89486]

Immunocytochemistry/Immunofluorescence: NIT2 Antibody [NBP1-89486] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & centrosome.
Immunohistochemistry-Paraffin: NIT2 Antibody [NBP1-89486]

Immunohistochemistry-Paraffin: NIT2 Antibody [NBP1-89486]

Immunohistochemistry-Paraffin: NIT2 Antibody [NBP1-89486] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
View 2 more images

Applications for NIT2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NIT2

NIT2 has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates byconverting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate andoxaloacetate, respectively. Overexpression decr

Alternate Names

EC 3.5.1.3, MGC111199, Nit protein 2, nitrilase family, member 2, Nitrilase homolog 2, omega-amidase NIT2

Gene Symbol

NIT2

Additional NIT2 Products

Product Documents for NIT2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NIT2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.