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NIT2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-17403

Novus Biologicals, part of Bio-Techne
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NBP3-17403-100ul
NBP3-17403-25ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for NIT2 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PGAFNLTTGPAHWELLQRSRAVDNQVYVATASPARDDKASYVAWGHSTVVNPWGEVLAKAGTEEAIVYSDIDLKKLAEIRQQIPVFRQKRSDLYAVEMK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NIT2 Antibody

Western Blot: NIT2 Antibody [NBP3-17403]

Western Blot: NIT2 Antibody [NBP3-17403]

Western Blot: NIT2 Antibody [NBP3-17403] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil
Immunohistochemistry-Paraffin: NIT2 Antibody [NBP3-17403]

Immunohistochemistry-Paraffin: NIT2 Antibody [NBP3-17403]

Immunohistochemistry-Paraffin: NIT2 Antibody [NBP3-17403] - Staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.

Applications for NIT2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NIT2

NIT2 has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates byconverting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate andoxaloacetate, respectively. Overexpression decr

Alternate Names

EC 3.5.1.3, MGC111199, Nit protein 2, nitrilase family, member 2, Nitrilase homolog 2, omega-amidase NIT2

Gene Symbol

NIT2

Additional NIT2 Products

Product Documents for NIT2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NIT2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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