Nitrilase 1 Antibody (1C3)
Novus Biologicals, part of Bio-Techne | Catalog # H00004817-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 1C3
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
NIT1 (NP_005591.1, 228 a.a. ~ 327 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AFGSITGPAHWEVLLRARAIETQCYVVAAAQCGRHHEKRASYGHSMVVDPWGTVVARCSEGPGLCLARIDLNYLRQLRRHLPVFQHRRPDLYGNLGHPLS
Specificity
Reacts with nitrilase 1.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Nitrilase 1 Antibody (1C3)
![Western Blot: Nitrilase 1 Antibody (1C3) [H00004817-M01]](https://resources.bio-techne.com/images/products/Nitrilase-1-Antibody-1C3-Western-Blot-H00004817-M01-img0005.jpg "Western Blot: Nitrilase 1 Antibody (1C3) [H00004817-M01]")
Western Blot: Nitrilase 1 Antibody (1C3) [H00004817-M01]
Western Blot: Nitrilase 1 Antibody (1C3) [H00004817-M01] - Analysis of NIT1 expression in transfected 293T cell line by NIT1 monoclonal antibody (M01), clone 1C3.Lane 1: NIT1 transfected lysate (Predicted MW: 35.9 KDa).Lane 2: Non-transfected lysate.![Immunocytochemistry/ Immunofluorescence: Nitrilase 1 Antibody (1C3) [H00004817-M01]](https://resources.bio-techne.com/images/products/Nitrilase-1-Antibody-1C3-Immunocytochemistry-Immunofluorescence-H00004817-M01-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Nitrilase 1 Antibody (1C3) [H00004817-M01]")
Immunocytochemistry/ Immunofluorescence: Nitrilase 1 Antibody (1C3) [H00004817-M01]
Immunocytochemistry/Immunofluorescence: Nitrilase 1 Antibody (1C3) [H00004817-M01] - Analysis of monoclonal antibody to NIT1 on HeLa cell . Antibody concentration 10 ug/ml.![Immunoprecipitation: Nitrilase 1 Antibody (1C3) [H00004817-M01]](https://resources.bio-techne.com/images/products/Nitrilase-1-Antibody-1C3-Immunoprecipitation-H00004817-M01-img0003.jpg "Immunoprecipitation: Nitrilase 1 Antibody (1C3) [H00004817-M01]")
Immunoprecipitation: Nitrilase 1 Antibody (1C3) [H00004817-M01]
Immunoprecipitation: Nitrilase 1 Antibody (1C3) [H00004817-M01] - Analysis of NIT1 transfected lysate using anti-NIT1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NIT1 monoclonal antibody.Applications for Nitrilase 1 Antibody (1C3)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in WB and ELISA.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Nitrilase 1
Alternate Names
EC 3.5, MGC57670, nitrilase 1, nitrilase homolog 1
Entrez Gene IDs
4817 (Human)
Gene Symbol
NIT1
UniProt
Additional Nitrilase 1 Products
Product Documents for Nitrilase 1 Antibody (1C3)
Product Specific Notices for Nitrilase 1 Antibody (1C3)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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