Nitrotyrosine Antibody (11C2)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-57383

Novus Biologicals, part of Bio-Techne
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NB100-57383
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Key Product Details

Species Reactivity

All Species

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 11C2

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Nitrotyrosine Antibodies »

Product Specifications

Immunogen

KLH-Nitrotyrosine

Reactivity Notes

There is no cross reaction with free or bound Tyrosine.

Specificity

The mouse monoclonal reacts with free and bound Nitrotyrosine

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for Nitrotyrosine Antibody (11C2)

Application
Recommended Usage

ELISA

1:100-1:1000

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:100-1:500

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Lyophilized powder, reconstituted in distilled water.

Formulation

Cell culture supernatant

Preservative

0.02% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nitrotyrosine

Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Various studies have shown that protein tyrosinenitration is limited to specific proteins and that the process is selective. For example, exposure of human surfactant protein A, SP-A, to oxygen-nitrogen intermediates generated by activated alveolar macrophages resulted in specific nitration of SP-A at tyrosines 164 and 166, while addition of 1.2 mMCO 2 resulted in additional nitration at tyrosine 161. The presence of nitrotyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimers disease, Parkinsons disease and amyotrophic lateral sclerosis.

Alternate Names

Nitrotyrosine

Additional Nitrotyrosine Products

Product Documents for Nitrotyrosine Antibody (11C2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Nitrotyrosine Antibody (11C2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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