Nitrotyrosine Antibody (EM-30) [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-96130CL1

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NBP1-96130AF350

Alexa Fluor 405

NBP1-96130AF405

Alexa Fluor 488

NBP1-96130AF488

Alexa Fluor 532

NBP1-96130AF532

Alexa Fluor 594

NBP1-96130AF594

Alexa Fluor 647

NBP1-96130AF647

Alexa Fluor 700

NBP1-96130AF700

Alexa Fluor 750

NBP1-96130AF750

Biotin

NBP1-96130B

DyLight 350

NBP1-96130UV

DyLight 405

NBP1-96130V

DyLight 488

NBP1-96130G

DyLight 550

NBP1-96130R

DyLight 594

NBP1-96130DL594

DyLight 650

NBP1-96130C

DyLight 680

NBP1-96130FR

DyLight 755

NBP1-96130IR

FITC

NBP1-96130F

HRP

NBP1-96130H

Janelia Fluor 525

NBP1-96130JF525

Janelia Fluor 549

NBP1-96130JF549

Janelia Fluor 646

NBP1-96130JF646

Janelia Fluor 669

NBP1-96130JF669

mFluor Violet 450 SE

NBP1-96130MFV450

mFluor Violet 500 SE

NBP1-96130MFV500

mFluor Violet 610 SE

NBP1-96130MFV610

PerCP

NBP1-96130PCP

Forumulation

Catalog #

BSA Free

NBP1-96130

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Non-species specific

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG₁ Clone # EM-30

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Nitrotyrosine Antibodies »

Product Specifications

Immunogen

NO2-Tyr-CH2-Thyroglobulin

Reactivity Notes

This antibody reacts to large number of species. Use in Mouse reported in secitific publication PMID: 32793221

Specificity

The antibody EM-30 detects nitrotyrosine group in biological material. It can be used as a marker for peroxynitrite formation in particular tissues

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for Nitrotyrosine Antibody (EM-30) [CoraFluor™ 1]

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Nitrotyrosine

Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitro tyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis (4).

Alternate Names

Nitrotyrosine

Additional Nitrotyrosine Products

Product Documents for Nitrotyrosine Antibody (EM-30) [CoraFluor™ 1]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for Nitrotyrosine Antibody (EM-30) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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