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NK1R Antibody (JE39-75)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32653

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32653

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE39-75

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant protein within Human NK1R aa 308-407 / 407. (Uniprot: P25103)

Localization

Cell membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NK1R Antibody (JE39-75)

NK1R Antibody (JE39-75)

Western Blot: NK1R Antibody (JE39-75) [NBP3-32653]

Western Blot: NK1R Antibody (JE39-75) [NBP3-32653] - Western blot analysis of NK1R on different lysates with Rabbit anti-NK1R antibody (NBP3-32653) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: SH-SY5Y cell lysate
Lane 3: U-87 MG cell lysate
Lane 4: NCCIT cell lysate
Lane 5: F9 cell lysate
Lane 6: Neuro-2a cell lysate
Lane 7: C6 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 46 kDa
Observed band size: 50 kDa

Exposure time: 20 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32653) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
NK1R Antibody (JE39-75)

Immunohistochemistry: NK1R Antibody (JE39-75) [NBP3-32653]

Immunohistochemistry: NK1R Antibody (JE39-75) [NBP3-32653] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-NK1R antibody (NBP3-32653) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32653) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
NK1R Antibody (JE39-75)

Immunocytochemistry/ Immunofluorescence: NK1R Antibody (JE39-75) [NBP3-32653]

Immunocytochemistry/ Immunofluorescence: NK1R Antibody (JE39-75) [NBP3-32653] - Immunocytochemistry analysis of Neuro-2a cells labeling NK1R with Rabbit anti-NK1R antibody (NBP3-32653) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NK1R antibody (NBP3-32653) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

Applications for NK1R Antibody (JE39-75)

Application
Recommended Usage

Flow Cytometry

1:500-1:1000

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry-Paraffin

1:200

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NK1R

The Neurokinins, also known as Tachykinins, belong to an evolutionary conserved family of peptide neurotransmitters that share a c-terminal sequence and have an established role in neurotransmission. The mammalian tachykinins include substance P (NK1), neurokinin A (NKA) and neurokinin B (NKB) which exert their effects by binding to specific receptors. Tachykinin peptides are important in the mediation of many physiological and pathological processes including inflammation, pain, migraine headache and allergy induced asthma.

Three tachykinin receptor types have been characterized, NK-1, NK-2 and NK-3 which have preferential affinities for SP, NKA and NKB respectively. All three receptors share a high degree of sequence homology, have seven transmembrane spanning domains and similar signal transduction mechanisms (e.g. G-protein coupled activation of phospholipase C).

Long Name

Neurokinin-1 Receptor

Alternate Names

SPR, TAC1R, TACR1

Gene Symbol

TACR1

Additional NK1R Products

Product Documents for NK1R Antibody (JE39-75)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NK1R Antibody (JE39-75)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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