NKCC2/SLC12A1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44269

Novus Biologicals, part of Bio-Techne
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NBP1-44269-0.025mg
NBP1-44269
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all NKCC2/SLC12A1 Antibodies »

Product Specifications

Immunogen

Produced against a synthetic peptide mapping to a segment (amino acids 33-55) of the N-terminal tail of rat NKCC2 (antibody desgination L224).

Localization

Membrane

Specificity

Detects approx 160kDa.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NKCC2/SLC12A1 Antibody

Western Blot: NKCC2/SLC12A1 Antibody [NBP1-44269]

Western Blot: NKCC2/SLC12A1 Antibody [NBP1-44269]

Western Blot: NKCC2/SLC12A1 Antibody [NBP1-44269] - Western blot analysis of Rat kidney tissue lysates showing detection of NKCC2/SLC12A1 protein using Rabbit Anti-NKCC2/SLC12A1 Polyclonal Antibody (NBP1-44269). Primary Antibody: Rabbit Anti-NKCC2/SLC12A1 Polyclonal Antibody (NBP1-44269) at 1:1000.
Immunohistochemistry: NKCC2/SLC12A1 Antibody [NBP1-44269]

Immunohistochemistry: NKCC2/SLC12A1 Antibody [NBP1-44269]

Immunohistochemistry: NKCC2/SLC12A1 Antibody [NBP1-44269] - Immunohistochemistry analysis using Rabbit Anti-NKCC2/SLC12A1 Polyclonal Antibody (NBP1-44269). Tissue: Kidney. Species: Rat. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-NKCC2/SLC12A1 Polyclonal Antibody (NBP1-44269) at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) NKCC2/SLC12A1 Antibody (D) Composite

Applications for NKCC2/SLC12A1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Western Blot

1:1000
Application Notes
1 ug/ml of NKCC2 Antibody was sufficient for detection of NKCC2 in 10 ug of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NKCC2/SLC12A1

NKCC2, a kidney-specific member of the cation chloride co-transporter family SLC12, facilitates transport of sodium, potassium, and chloride ions from the lumen of the renal thick ascending limb into the cell (1, 2). Transport of sodium dilutes the luminal fluid, decreasing its osmolality creating an osmotic driving force for water reabsorption in the connecting tubule and cortical collecting duct under the influence of the hormone vasopressin. NKCC2 is found on the apical surface of the thick ascending limb of the loop of Henle and is blocked by loop diuretics such as furosemide (1).

Long Name

Sodium/Potassium/Chloride Transporter NKCC2

Alternate Names

BSC1, SLC12A1

Entrez Gene IDs

6557 (Human); 25065 (Rat)

Gene Symbol

SLC12A1

UniProt

Q5PQV1 (Rat)

Additional NKCC2/SLC12A1 Products

Product Documents for NKCC2/SLC12A1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NKCC2/SLC12A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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