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NKD1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-55270

Novus Biologicals, part of Bio-Techne
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NBP1-55270

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Synthetic peptide directed towards the N terminal of human NKD1 (NP_149110). Peptide sequence: ELVGDVLRDTLSEEEEDDFRLEVALPPEKTDGLGSGDEKKMERVSEPCPG. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for NKD1 Antibody

Western Blot: NKD1 Antibody [NBP1-55270]

Western Blot: NKD1 Antibody [NBP1-55270]

Western Blot: NKD1 Antibody [NBP1-55270] - HepG2 tissue lysate at a concentration of 1ug/ml.
Immunohistochemistry-Paraffin: NKD1 Antibody [NBP1-55270]

Immunohistochemistry-Paraffin: NKD1 Antibody [NBP1-55270]

Immunohistochemistry-Paraffin: NKD1 Antibody [NBP1-55270] - Human Lung Alveolar cells (indicated with arrows), 4-8ug/ml.

Applications for NKD1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

4-8 ug/ml

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NKD1

In the mouse, Nkd is a Dishevelled -binding protein that functions as a negative regulator of the Wnt-beta-catenin -Tcf signaling pathway.In the mouse, Nkd is a Dishevelled (see DVL1; MIM 601365)-binding protein that functions as a negative regulator of the Wnt (see WNT1; MIM 164820)-beta-catenin (see MIM 116806)-Tcf (see MIM 602272) signaling pathway.[supplied by OMIM].

Alternate Names

Dvl-binding protein, hNkd, hNkd1, naked cuticle homolog 1 (Drosophila), naked cuticle-1, Naked1, Naked-1, NKD, protein naked cuticle homolog 1

Entrez Gene IDs

85407 (Human)

Gene Symbol

NKD1

UniProt

Additional NKD1 Products

Product Documents for NKD1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NKD1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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