Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1D11

Concentration

1.0 mg/ml

View all NKG2D/CD314 Antibodies »

Product Specifications

Immunogen

NKL cells

Specificity

NB100-65956 is specific for CD314, also known as natural killer receptor G2 (NKG2D) and as killer cell lectin-like receptor subfamily K, member 1 (KLRK1). CD314 is a C-type lectin-like activating receptor which is expressed on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. CD314 forms homodimers that signal through an associated DAP10 adaptor protein. Ligands of CD314 include MICA, MICB and UL16 binding protein (ULBP), which are inducibly expressed. Ligand binding to CD314 results in NK cell activation and potent costimulation of effector T cells. Clone 1D11 is reported to inhibit T cell recognition of MICA (1,6).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for NKG2D/CD314 Antibody (1D11)

Immunohistochemistry-Paraffin: NKG2D/CD314 Antibody (1D11) - Low Endotoxin, Azide and BSA Free [NB100-65956]

Immunohistochemistry-Paraffin: NKG2D/CD314 Antibody (1D11) - Low Endotoxin, Azide and BSA Free [NB100-65956]

Immunohistochemistry-Paraffin: NKG2D/CD314 Antibody (1D11) - Low Endotoxin [NB100-65956] - Analysis of human tonsil tissue using NKG2D/CD314 antibody. PFA fixed paraffin-embedded sections. Heat-mediated antigen retrieval was performed for 30 minutes in citrate buffer. Primary antibody dilution was 1:200. Image from verified customer review.
Flow Cytometry: NKG2D/CD314 Antibody (1D11) - Low Endotoxin, Azide and BSA Free [NB100-65956]

Flow Cytometry: NKG2D/CD314 Antibody (1D11) - Low Endotoxin, Azide and BSA Free [NB100-65956]

Flow Cytometry: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Analysis using the Biotin conjugate of NB100-65956. Staining of Human peripheral blood lymphocytes with biotinylated 1D11, followed by Sav-PE.
Immunohistochemistry-Frozen: NKG2D/CD314 Antibody (1D11) - Low Endotoxin, Azide and BSA Free [NB100-65956]

Immunohistochemistry-Frozen: NKG2D/CD314 Antibody (1D11) - Low Endotoxin, Azide and BSA Free [NB100-65956]

Immunohistochemistry-Frozen: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Immunoperoxidase staining of a human spleen cryosection with Mouse anti Human CD314 antibody,
View 6 more images

Applications for NKG2D/CD314 Antibody (1D11)

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500
Application Notes
Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24232187). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27759906). For flow, recommended use is 10ul of suggessted working dilution to label 10^6 cells in 100ul.

Reviewed Applications

Read 1 review rated 5 using NB100-65956 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NKG2D/CD314

Natural killer group 2 member D (NKG2D), also known as killer lectin-like receptor subfamily K member 1 (KLRK1) and CD314, is a 42 kDa type II transmembrane protein belonging to the C-type lectin superfamily and is an activating receptor for NK cells (1). NKG2D is expressed on innate and adaptive immune cells such as NK cells, NKT cells, CD8+ T cells, and gammadelta T cells (1-4). Under pathological conditions, including Crohn's disease and Rheumatoid arthritis, NKG2D expression can be induced on CD4+ cells (2,3). NKG2D is synthesized as a protein of 216 amino acids (aa) in length with a theoretical molecular weight of 25.3 kDa (5). The full NKG2D receptor is expressed on the cell surface as a disulfide-linked homodimer and, in humans, has several ligands expressed on tumor cells including MHC class I-related genes A and B (MICA and MICB) and 6 members of the UL16-binding protein (ULBP) family (1-4, 6). NKG2D interaction with its ligand results in activation of NK cells via the recruitment of adapter molecules DNAX-activating protein of 10 kDa (DAP10) or DAP12 homodimers to the intracellular tail region of NKG2D (1-4, 6). In humans, NKG2D can only bind DAP10; however, mice have both short and long NKG2D isoforms which can also bind DAP12 (2-4, 6). Following NKG2D receptor and ligand interaction, the YXXM motif of the DAP10 adapter molecule initiates a downstream signaling cascade through Grb2 and PI3K pathways, resulting in NK cell cytotoxicity (4,6).

In general, NKG2D signaling has dual functions, playing a role in both immune surveillance and immune escape (4,6). Detection of viruses or pathogens and corresponding cytokine production induces NKG2D-ligand expression on dendritic cells and macrophages as well as NKG2D receptor upregulation on NK cells to initiate an immune response (1,2,4,6). Similarly, NKG2D-ligand expression on tumor cells is upregulated in cancers such as liver cancer, ovarian cancer, colon cancer, and leukemia, and the NKG2D/NKG2D-ligand signaling axis functions in preventing progression and metastasis (1,4,6). By contrast, NKG2D signaling also mediates tumor escape through ligand shedding via proteases from the matrix metalloproteinase (MMP) and a disintegrin and metalloprotease (ADAM) families and transforming growth factor (TGF)-beta inhibition of T cell and NK cell function (4). A number of therapeutic strategies targeting the NKG2D receptor-ligand pathway have been developed for cancer immunotherapies including upregulating NKG2D expression on immune cells via soluble cytokines, modulating ligand expression with histone deacetylase (HDAC) inhibitors, or inhibiting ligand shedding molecules like ADAMs and MMPs (1,4,6). Alternatively, in instances of autoimmune diseases or inflammation, NKG2D blocking strategies are of interest (6).

References

1. Wang, J., Li, C. D., & Sun, L. (2020). Recent Advances in Molecular Mechanisms of the NKG2D Pathway in Hepatocellular Carcinoma. Biomolecules, 10(2), 301. https://doi.org/10.3390/biom10020301

2. Stojanovic, A., Correia, M. P., & Cerwenka, A. (2018). The NKG2D/NKG2DL Axis in the Crosstalk Between Lymphoid and Myeloid Cells in Health and Disease. Frontiers in immunology, 9, 827. https://doi.org/10.3389/fimmu.2018.00827

3. Wensveen, F. M., Jelencic, V., & Polic, B. (2018). NKG2D: A Master Regulator of Immune Cell Responsiveness. Frontiers in immunology, 9, 441. https://doi.org/10.3389/fimmu.2018.00441

4. Liu, H., Wang, S., Xin, J., Wang, J., Yao, C., & Zhang, Z. (2019). Role of NKG2D and its ligands in cancer immunotherapy. American journal of cancer research, 9(10), 2064-2078.

5. Uniprot (P26718)

6. Lanier L. L. (2015). NKG2D Receptor and Its Ligands in Host Defense. Cancer immunology research, 3(6), 575-582. https://doi.org/10.1158/2326-6066.CIR-15-0098

Long Name

Natural Killer G2D

Alternate Names

CD314, D12S2489E, KLRK1

Gene Symbol

KLRK1

UniProt

P26718

Additional NKG2D/CD314 Products

Product Documents for NKG2D/CD314 Antibody (1D11)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NKG2D/CD314 Antibody (1D11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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