NKX2.2 Antibody (NX2/294) - IHC-Prediluted

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45154

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Chicken

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # NX2/294

Format

IHC-Prediluted

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NKX2.2 Antibodies »

Product Specifications

Immunogen

Human full-length recombinant NKX2.2 protein (Uniprot: O95096)

Localization

Nuclear

Marker

Neuroendocrine & Ewing's Sarcoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for NKX2.2 Antibody (NX2/294) - IHC-Prediluted

Immunohistochemistry-Paraffin: NKX2.2 Antibody (NX2/294) - IHC-Prediluted [NBP2-45154]

Immunohistochemistry-Paraffin: NKX2.2 Antibody (NX2/294) - IHC-Prediluted [NBP2-45154]

Immunohistochemistry-Paraffin: NKX2.2 Antibody (NX2/294) - IHC-Prediluted [NBP2-45154] - Formalin-fixed, paraffin-embedded Rat Pancreas stained with NKX2.2 Antibody (NX2/294).
Immunohistochemistry-Frozen: NKX2.2 Antibody (NX2/294) - IHC-Prediluted [NBP2-45154]

Immunohistochemistry-Frozen: NKX2.2 Antibody (NX2/294) - IHC-Prediluted [NBP2-45154]

Immunohistochemistry-Frozen: NKX2.2 Antibody (NX2/294) - IHC-Prediluted [NBP2-45154] - Analysis of NKX2.2 in human pancreas frozen tissue section using NKX2.2 antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: NKX2.2 Antibody (NX2/294) - IHC-Prediluted [NBP2-45154]

Immunohistochemistry-Paraffin: NKX2.2 Antibody (NX2/294) - IHC-Prediluted [NBP2-45154]

Immunohistochemistry-Paraffin: NKX2.2 Antibody (NX2/294) - IHC-Prediluted [NBP2-45154] - Formalin-fixed, paraffin-embedded human Ewing's Sarcoma stained with NKX2.2 Antibody (NX2/294).
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Applications for NKX2.2 Antibody (NX2/294) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: NKX2.2

Nkx2.2, or Homeobox protein Nkx-2.2, consists of a 273 amino acid isoform that is 30 kDa, and is involved in controlling and maintaining the expression of genes within pancreatic endocrine cells and axons. Current research is being conducted on the relationship between Nkx2.2 and a variety of diseases and disorders, including glioma, neuronitis, maturity-onset diabetes, pancreatitis, Partington syndrome, sarcoma, gastrointestinal neuroendocrine tumor, acute lymphoblastic leukemia, multiple sclerosis, and carcinoid tumors. The protein has been linked to the Wnt, Hedgehog, and notch pathways, as well as regulation of beta-cell development and gene expression. NKx2.2 interacts with several other proteins, including OLIG2, HDAC1, SIN3A, FOXA2, and FOXO1.

Long Name

NK2 Homeobox 2

Alternate Names

NKX2B

Gene Symbol

NKX2-2

Additional NKX2.2 Products

Product Documents for NKX2.2 Antibody (NX2/294) - IHC-Prediluted

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NKX2.2 Antibody (NX2/294) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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