NKX2.2 Antibody (SPM564)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32884

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-32884-0.02mg
NBP2-32884-0.1mg
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat, Chicken

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # SPM564

Concentration

0.2 mg/ml

View all NKX2.2 Antibodies »

Product Specifications

Immunogen

Human NKX2.2 recombinant protein (Uniprot: O95096)

Localization

Nuclear

Marker

Neuroendocrine & Ewing Sarcoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34799)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for NKX2.2 Antibody (SPM564)

Immunohistochemistry-Paraffin: NKX2.2 Antibody (SPM564) [NBP2-32884]

Immunohistochemistry-Paraffin: NKX2.2 Antibody (SPM564) [NBP2-32884]

Immunohistochemistry-Paraffin: NKX2.2 Antibody (SPM564) [NBP2-32884] - Formalin-fixed, paraffin-embedded human pancreas stained with NKX2.2 Monoclonal Antibody (SPM564).
Immunohistochemistry-Paraffin: NKX2.2 Antibody (SPM564) [NBP2-32884]

Immunohistochemistry-Paraffin: NKX2.2 Antibody (SPM564) [NBP2-32884]

Immunohistochemistry-Paraffin: NKX2.2 Antibody (SPM564) [NBP2-32884] - Formalin-paraffin Ewings sarcoma stained with NKX2.2 MAb (SPM564).

Applications for NKX2.2 Antibody (SPM564)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: NKX2.2

Nkx2.2, or Homeobox protein Nkx-2.2, consists of a 273 amino acid isoform that is 30 kDa, and is involved in controlling and maintaining the expression of genes within pancreatic endocrine cells and axons. Current research is being conducted on the relationship between Nkx2.2 and a variety of diseases and disorders, including glioma, neuronitis, maturity-onset diabetes, pancreatitis, Partington syndrome, sarcoma, gastrointestinal neuroendocrine tumor, acute lymphoblastic leukemia, multiple sclerosis, and carcinoid tumors. The protein has been linked to the Wnt, Hedgehog, and notch pathways, as well as regulation of beta-cell development and gene expression. NKx2.2 interacts with several other proteins, including OLIG2, HDAC1, SIN3A, FOXA2, and FOXO1.

Long Name

NK2 Homeobox 2

Alternate Names

NKX2B

Gene Symbol

NKX2-2

UniProt

O95096 (Human)

Additional NKX2.2 Products

Product Documents for NKX2.2 Antibody (SPM564)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NKX2.2 Antibody (SPM564)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.