NKX2.2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82554

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-82554
NBP1-82554-25ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Feline

Cited:

Human, Mouse

Predicted:

Rat (97%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin, Knockout Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NKX2.2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SLTNTKTGFSVKDILDLPDTNDEEGSVAEGPEEENEGPEPAKRAGPLGQGALDAVQSLPLKNPFYDSSDNPYTRWLASTEGLQYSLHGLAAGAPPQDSSSKSPEPSADESPDNDKETP

Reactivity Notes

Feline, Mouse reactivity reported from verified customer reviews.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NKX2.2 Antibody

Western Blot: NKX2.2 Antibody [NBP1-82554]

Western Blot: NKX2.2 Antibody [NBP1-82554]

Western Blot: NKX2.2 Antibody [NBP1-82554] - Analysis in human cell line U-138 MG.
Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP1-82554]

Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP1-82554]

Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP1-82554] - Staining of human cerebellum shows moderate postivity in cells in molecular layer.
Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP1-82554]

Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP1-82554]

Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP1-82554] - Staining of human pancreas shows strong nuclear positivity in islet cells.
View 2 more images

Applications for NKX2.2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Validated from verified customer review.

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Frozen

Validated from verified customer review.

Immunohistochemistry-Paraffin

1:50 - 1:200

Knockout Validated

Validated (bioRxiv Jan 1 2020)

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 2 reviews rated 4 using NBP1-82554 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NKX2.2

Nkx2.2, or Homeobox protein Nkx-2.2, consists of a 273 amino acid isoform that is 30 kDa, and is involved in controlling and maintaining the expression of genes within pancreatic endocrine cells and axons. Current research is being conducted on the relationship between Nkx2.2 and a variety of diseases and disorders, including glioma, neuronitis, maturity-onset diabetes, pancreatitis, Partington syndrome, sarcoma, gastrointestinal neuroendocrine tumor, acute lymphoblastic leukemia, multiple sclerosis, and carcinoid tumors. The protein has been linked to the Wnt, Hedgehog, and notch pathways, as well as regulation of beta-cell development and gene expression. NKx2.2 interacts with several other proteins, including OLIG2, HDAC1, SIN3A, FOXA2, and FOXO1.

Long Name

NK2 Homeobox 2

Alternate Names

NKX2B

Entrez Gene IDs

4821 (Human)

Gene Symbol

NKX2-2

UniProt

O95096 (Human)

Additional NKX2.2 Products

Product Documents for NKX2.2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NKX2.2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.