Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all NKX2.6 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 15 amino acid synthetic peptide near the center of human NKX2-6. The immunogen is located within amino acids 170 - 220 of NKX2-6. Amino Acid Squence: KRQRQDKSLELAGH

Specificity

NKX2-6 antibody is predicted to not cross-react with other NK2 homeobox family members. At least two isoforms of NKX2-6 are known to exist; this antibody will detect both isoforms

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NKX2.6 Antibody - BSA Free

Western Blot: NKX2.6 AntibodyBSA Free [NBP2-41161]

Western Blot: NKX2.6 AntibodyBSA Free [NBP2-41161]

Western Blot: NKX2.6 Antibody [NBP2-41161] - Analysis of NKX2-6 in mouse heart tissue lysate with NKX2-6 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
NKX2.6 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: NKX2.6 Antibody - BSA Free [NBP2-41161] -

Immunocytochemistry/ Immunofluorescence: NKX2.6 Antibody - BSA Free [NBP2-41161] - Immunofluorescence of NKX-6 in human spleen tissue with NKX2.6 antibody at 20 ug/mL.
NKX2.6 Antibody - BSA Free

Immunohistochemistry: NKX2.6 Antibody - BSA Free [NBP2-41161] -

Immunohistochemistry: NKX2.6 Antibody - BSA Free [NBP2-41161] - Immunohistochemistry of NKX2.6 in human spleen tissue with NKX2.6 antibody at 5 ug/mL.

Applications for NKX2.6 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5 ug/mL

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NKX2.6

Members of the NK-2 family of homeodomain proteins are key regulators of growth and development in several tissues, including brain, heart and pancreas.Nkx-2.5, also designated cardiac specific homeobox protein (Csx), is a homolog of the Drosophila tinman protein and is essential for normal cardiovascular development. Expression of Nkx-2.5 during cardiomyogenesis is required for cardiac septation, in which a single atrium and ventricle are separated into four chambers. Nkx-2.5 binds to DNA as a monomer, a homodimer or as a heterodimer with Nkx-2.3 or Nkx-2.6, which suggests that the specific protein-protein interactions of Nkx-2.5 are involved in its transcriptional regulatory function. Nkx-2.6, also a homolog of the Drosophila tinman protein, is expressed in the caudal pharyngeal pouches,the caudal heart progenitors, the sinus venosus, the outflow tract of the heart and in a short segment of the gut between stages E8.5 and E10.5 of embryogenesis. Expression of Nkx-2.6 overlaps with that of Nkx-2.5 in the pharynx and heart. However, Nkx-2.6 mutant mice are viable and fertile, which suggests that Nkx-2.6 plays a compensatory function to Nkx-2.5.

Alternate Names

CSX2, NK2 homeobox 6, NKX2F, NKX4-2

Gene Symbol

NKX2-6

Additional NKX2.6 Products

Product Documents for NKX2.6 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NKX2.6 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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