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NKX3.1 Antibody (0361) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80880

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 0361

Format

Azide and BSA Free

Concentration

2.3 mg/ml

Product Specifications

Immunogen

Full-length human Nkx3.1 produced in bacteria as a hexahistidine-tagged protein. [UniProt# Q99801]

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 22465996).

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for NKX3.1 Antibody (0361) - Azide and BSA Free

Western Blot: NKX3.1 Antibody (0361)Azide and BSA Free [NBP2-80880]

Western Blot: NKX3.1 Antibody (0361)Azide and BSA Free [NBP2-80880]

Western Blot: NKX3.1 Antibody (0361) - Azide and BSA Free [NBP2-80880] - Detection of NKX3.1 in mouse testis lysate using NB 100-1828. ECL detection 1 minute. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: NKX3.1 Antibody (0361) - Azide and BSA Free [NBP2-80880]

Immunohistochemistry-Paraffin: NKX3.1 Antibody (0361) - Azide and BSA Free [NBP2-80880]

Immunohistochemistry-Paraffin: NKX3.1 Antibody (0361) - Azide and BSA Free [NBP2-80880] - NKX3.1 was detected in immersion fixed paraffin-embedded sections of mouse testis using Mouse Anti-Mouse NKX3.1 (0361) Monoclonal Antibody (Catalog # NB100-1828) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCy
Western Blot: NKX3.1 Antibody (0361)Azide and BSA Free [NBP2-80880]

Western Blot: NKX3.1 Antibody (0361)Azide and BSA Free [NBP2-80880]

Western Blot: NKX3.1 Antibody (0361) - Azide and BSA Free [NBP2-80880] - analysis of NKX3.1 in HeLa cell lysate using anti-NKX3.1 antibody. Image from verified customer review. Image from the standard format of this antibody.

Applications for NKX3.1 Antibody (0361) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100

Immunohistochemistry-Frozen

1:100

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1:10-1:500

Western Blot

2 ug/ml
Application Notes
This Nkx3.1 antibody is useful for Immunohistochemistry frozen and paraffin embedded sections, Immunoprecipitation and Western Blot.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

2.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NKX3.1

NKX3.1 (homeobox protein Nkx-3.1) is a homeodomain nuclear transcription factor expressed predominately in the prostate with very low testicular expression and is highly important in prostate biology. NKX3.1 interacts with SRF, SPDEF, WDR77 etc. and TOPORS polyubiquitinates NKX3-1 for inducing its proteasomal degradation regulation. NKX3.1 binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and act as a transcriptional repressor for playing important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Additionally, NKX3.1 functions as a prostate-specific tumor suppressor and diminished NKX3.1 expression is a consistent feature in human/mouse prostate cancers, wherein its expression is lost early in tumorigenesis suggesting that NKX3.1 loss is an early step in the progression to malignant disease.

Long Name

NK3 Homeobox 1

Alternate Names

BAPX2, NKX3-1, NKX3A

Gene Symbol

NKX3-1

Additional NKX3.1 Products

Product Documents for NKX3.1 Antibody (0361) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NKX3.1 Antibody (0361) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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