NM23-H2/NME2 Antibody (CPTC-NME2-2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79738

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # CPTC-NME2-2

Concentration

0.2 mg/ml

View all NM23-H2 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human NM23-H2/NME2 protein (Uniprot: P22392)

Localization

Cytoplasmic, Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79873)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for NM23-H2/NME2 Antibody (CPTC-NME2-2)

Western Blot: NM23-H2/NME2 Antibody (CPTC-NME2-2) [NBP2-79738]

Western Blot: NM23-H2/NME2 Antibody (CPTC-NME2-2) [NBP2-79738]

Western Blot: NM23-H2/NME2 Antibody (CPTC-NME2-2) [NBP2-79738] - Western Blot Analysis of PC-3 cell lysate using NM23-H2/NME2 Antibody (CPTC-NME2-2).
Immunohistochemistry-Paraffin: NM23-H2/NME2 Antibody (CPTC-NME2-2) [NBP2-79738]

Immunohistochemistry-Paraffin: NM23-H2/NME2 Antibody (CPTC-NME2-2) [NBP2-79738]

Immunohistochemistry-Paraffin: NM23-H2/NME2 Antibody (CPTC-NME2-2) [NBP2-79738] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with NM23-H2/NME2 Antibody (CPTC-NME2-2).
Protein Array: NM23-H2/NME2 Antibody (CPTC-NME2-2) [NBP2-79738]

Protein Array: NM23-H2/NME2 Antibody (CPTC-NME2-2) [NBP2-79738]

Protein Array: NM23-H2/NME2 Antibody (CPTC-NME2-2) [NBP2-79738] - Analysis of Protein Array containing more than 19,000 full-length human proteins using NM23-H2/NME2 Antibody (CPTC-NME2-2). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Applications for NM23-H2/NME2 Antibody (CPTC-NME2-2)

Application
Recommended Usage

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: NM23-H2

Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene. Co-transcription of this gene and the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) which encode a fusion protein comprised of sequence sharing identity with each individual gene product.

Long Name

Non-metastatic Protein 23 Homolog 2

Alternate Names

NDK B, NDP Kinase B2, NDPKB, NM23B, NME2

Gene Symbol

NME2

UniProt

P22392

Additional NM23-H2 Products

Product Documents for NM23-H2/NME2 Antibody (CPTC-NME2-2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NM23-H2/NME2 Antibody (CPTC-NME2-2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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