Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # S327-95

Concentration

1 mg/ml

View all NMDAR2A Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 75-325 (extracellular N-terminus) of rat GluN2A/NR2A

Localization

Cell Membrane , Cell Junction

Specificity

Detects approx 170kDa. Does not react with NR2B.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for NMDAR2A Antibody (S327-95)

Western Blot: NMDAR2A Antibody (S327-95) [NBP2-22405]

Western Blot: NMDAR2A Antibody (S327-95) [NBP2-22405]

Western Blot: NMDAR2A Antibody (S327-95) [NBP2-22405] - Western Blot analysis of Monkey COS cells transfected with GFP-tagged NMDAR2A showing detection of ~170 kDa NMDAR2A protein using Mouse Anti-NMDAR2A Monoclonal Antibody, Clone S327-95 (NBP2-22405). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged NMDAR2A. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-NMDAR2A Monoclonal Antibody (NBP2-22405) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~170 kDa. Other Band(s): 100 kDa.
Immunocytochemistry/ Immunofluorescence: NMDAR2A Antibody (S327-95) [NBP2-22405]

Immunocytochemistry/ Immunofluorescence: NMDAR2A Antibody (S327-95) [NBP2-22405]

Immunocytochemistry/Immunofluorescence: NMDAR2A Antibody (S327-95) [NBP2-22405] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2A/NR2A Monoclonal Antibody, Clone S327-95 (NBP2-22405). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GluN2A/NR2A Monoclonal Antibody (NBP2-22405) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GluN2A/NR2A Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: NMDAR2A Antibody (S327-95) [NBP2-22405]

Immunocytochemistry/ Immunofluorescence: NMDAR2A Antibody (S327-95) [NBP2-22405]

Immunocytochemistry/Immunofluorescence: NMDAR2A Antibody (S327-95) [NBP2-22405] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GluN2A/NR2A Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Cell Junction. Magnification: 60X.
View 1 more images

Applications for NMDAR2A Antibody (S327-95)

Application
Recommended Usage

Western Blot

1:1000
Application Notes
1 ug/ml of GluN2A/NR2A Antibody was sufficient for detection o GluN2A/NR2A in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NMDAR2A

The ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning. NMDA receptors are also implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. NMDA receptors also appear to be a target for ethanol at physiological concentrations and therefore may play a significant role in alcoholism.

Alternate Names

GluN2A, glutamate receptor, ionotropic, N-methyl D-aspartate 2A, hNR2A, NMDA receptor subtype 2A, N-methyl D-aspartate receptor subtype 2A, N-methyl-D-aspartate receptor subunit 2A, subunit epsilon-1

Gene Symbol

GRIN2A

UniProt

Q00959 (Rat)

Additional NMDAR2A Products

Product Documents for NMDAR2A Antibody (S327-95)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NMDAR2A Antibody (S327-95)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.