NMDAR2A [p Tyr1325] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29535

Novus Biologicals, part of Bio-Techne
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NBP2-29535
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Hamster (100%), Sheep (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NMDAR2A Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1325 conjugated to KLH. Accession # Q00959

Modification

p Tyr1325

Specificity

Specific for endogenous levels of the ~180k NMDAR NR2A-subunit protein phosphorylated at Tyr1325 in Western blots. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

180 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NMDAR2A [p Tyr1325] Antibody

Western Blot: NMDAR2A [p Tyr1325] Antibody [NBP2-29535]

Western Blot: NMDAR2A [p Tyr1325] Antibody [NBP2-29535]

Western Blot: NMDAR2A [p Tyr1325] Antibody [NBP2-29535] - Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2A subunit of the NMDAR phosphorylated at Tyr1325 (Control). The phosphospecificity of this labeling is shown in the second lane where immunoreactivity is blocked by preadsorption with the phospho-peptide (Peptide) used as antigen but not by the dephosphopeptide (not shown).

Applications for NMDAR2A [p Tyr1325] Antibody

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NMDAR2A

The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). Channels with physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Recently, phosphorylation of Tyrosine 1325 of the NR2A subunit has been shown to be increased in human brain tissue sections from HIV-infected individuals with encephalitis (King et al., 2010). In addition, Tyr1325 phosphorylation has been linked with depression-related behavior (Taniguchi et al., 2009).

Alternate Names

GluN2A, glutamate receptor, ionotropic, N-methyl D-aspartate 2A, hNR2A, NMDA receptor subtype 2A, N-methyl D-aspartate receptor subtype 2A, N-methyl-D-aspartate receptor subunit 2A, subunit epsilon-1

Entrez Gene IDs

24409 (Rat)

Gene Symbol

GRIN2A

UniProt

Q00959 (Rat)

Additional NMDAR2A Products

Product Documents for NMDAR2A [p Tyr1325] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NMDAR2A [p Tyr1325] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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