Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Bovine (100%), Orangutan (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all NMT1 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 68 to 118 of human N-Myristoyltransferase 1 using the numbering given in entry NP_066565.1 (GeneID 4836).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NMT1 Antibody

Western Blot: NMT1 Antibody [NBP2-32168]

Western Blot: NMT1 Antibody [NBP2-32168]

Western Blot: NMT1 Antibody [NBP2-32168] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-NMT1 antibody NBP2-32168 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: NMT1 Antibody [NBP2-32168]

Immunoprecipitation: NMT1 Antibody [NBP2-32168]

Immunoprecipitation: NMT1 Antibody [NBP2-32168] - Detection of human NMT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 to 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-NMT1 antibody NBP2-32168 used for IP at 6 ug per reaction. NMT1 was also immunoprecipitated by rabbit anti-NMT1 antibody NBP2-32167. For blotting immunoprecipitated NMT1, NBP2-32168 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Knockdown Validated: NMT1 Antibody [NBP2-32168]

Knockdown Validated: NMT1 Antibody [NBP2-32168]

Knockdown Validated: NMT1 Antibody [NBP2-32168] - NMT1 activity is necessary to maintain the autophagic flux in cancer cells. NMT1 expression in control and NMT1 KD H460 clones. Numbers under each lane, densitometry values (arbitrary units) for NMT1 signal normalized to actin in one representative experiment from two independent experiments. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-020-68615-w), licensed under a CC-BY license.

Applications for NMT1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Reported in scientific publication (PMID: 32686708)

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NMT1

Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT; EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1; MIM 139311) (Duronio et al., 1992 (PubMed 1570339)).(supplied by OMIM)

Alternate Names

alternative, short form NMT-S, glycylpeptide N-tetradecanoyltransferase 1, long form, NMT-L, Myristoyl-CoA:protein N-myristoyltransferase 1, NMT 1, NMTEC 2.3.1.97, N-myristoyltransferase 1, Peptide N-myristoyltransferase 1, Type I N-myristoyltransferase

Gene Symbol

NMT1

UniProt

NP_066565.1 (Human)

Additional NMT1 Products

Product Documents for NMT1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NMT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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