nNOS Antibody (5K9R2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16008

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5K9R2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all nNOS Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1335-1434 of human nNOS (P29475). QLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSAEDAGVFISRMRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for nNOS Antibody (5K9R2)

Western Blot: nNOS Antibody (5K9R2) [NBP3-16008]

Western Blot: nNOS Antibody (5K9R2) [NBP3-16008]

Western Blot: nNOS Antibody (5K9R2) [NBP3-16008] - Western blot analysis of extracts of Rat brain, using nNOSantibody (NBP3-16008) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Immunocytochemistry/ Immunofluorescence: nNOS Antibody (5K9R2) [NBP3-16008]

Immunocytochemistry/ Immunofluorescence: nNOS Antibody (5K9R2) [NBP3-16008]

Immunocytochemistry/Immunofluorescence: nNOS Antibody (5K9R2) [NBP3-16008] - Immunofluorescence analysis of SH-SY5Y cells using nNOSRabbit mAb (NBP3-16008) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: nNOS Antibody (5K9R2) [NBP3-16008]

Immunocytochemistry/ Immunofluorescence: nNOS Antibody (5K9R2) [NBP3-16008]

Immunocytochemistry/Immunofluorescence: nNOS Antibody (5K9R2) [NBP3-16008] - Immunofluorescence analysis of Neuro-2a cells using nNOSRabbit mAb (NBP3-16008) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Applications for nNOS Antibody (5K9R2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: nNOS

NOS (nitric oxide synathase) is a mediator of NO (nitric oxide) production, an inorganic gaseous messenger between cells. In cells, NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Three isoforms of NOS has been identified (eNOS, iNOS, and nNOS) with all of them having binding sites for NADPH, FAD, FMN, and tetrahydrobiopterin (1). Specifically, nNOS (Neuronal nitric oxide synthase bNOS, cNOS, Type I) is bound to PSD95 located in the neuronal membrane and at increased level of Ca2+, nNOS is trasnlocated to cytoplasm. In cytoplasm, nNOS is dephosphorylated by calcineurin which initiates NO production (2). A phosphorylation by PKA and PKC on nNOS leads down-regulation of NO production.

Long Name

Neuronal Nitric Oxide Synthase

Alternate Names

NOS1

Gene Symbol

NOS1

Additional nNOS Products

Product Documents for nNOS Antibody (5K9R2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for nNOS Antibody (5K9R2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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