Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

ICC/IF (Negative), Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 60718

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all nNOS Antibodies »

Product Specifications

Immunogen

Partial recombinant human nNOS protein (amino acids 218-1434) [UniProt P29475]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

>90% by SDS PAGE

Scientific Data Images for nNOS Antibody (60718) - Azide and BSA Free

Western Blot: nNOS Antibody (60718)Azide and BSA Free [MAB2416]

Western Blot: nNOS Antibody (60718)Azide and BSA Free [MAB2416]

Western Blot: nNOS Antibody (60718) [MAB2416] - Analysis of mouse and rat brain Tissue lysate with ug/ml of hnNOS Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hnNOS at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
nNOS Antibody (60718) - Azide and BSA Free

Western Blot: nNOS Antibody (60718) [MAB2416] - 

Western Blot: nNOS Antibody (60718) [MAB2416] - Effects of IFN alpha and IFN gamma treatment on nNOS expression levels in human metastatic melanoma A375 cells. Cells were treated with IFN alpha or IFN gamma for various timepoints and whole cell lysates were collected. Samples were subjected to Western blot analysis for nNOS. A control of the protein loading was performed by detecting actin. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35105915/) licensed under a CC-BY license.

Applications for nNOS Antibody (60718) - Azide and BSA Free

Application
Recommended Usage

ICC/IF (Negative)

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: nNOS

NOS (nitric oxide synathase) is a mediator of NO (nitric oxide) production, an inorganic gaseous messenger between cells. In cells, NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Three isoforms of NOS has been identified (eNOS, iNOS, and nNOS) with all of them having binding sites for NADPH, FAD, FMN, and tetrahydrobiopterin (1). Specifically, nNOS (Neuronal nitric oxide synthase bNOS, cNOS, Type I) is bound to PSD95 located in the neuronal membrane and at increased level of Ca2+, nNOS is trasnlocated to cytoplasm. In cytoplasm, nNOS is dephosphorylated by calcineurin which initiates NO production (2). A phosphorylation by PKA and PKC on nNOS leads down-regulation of NO production.

Long Name

Neuronal Nitric Oxide Synthase

Alternate Names

NOS1

Gene Symbol

NOS1

Additional nNOS Products

Product Documents for nNOS Antibody (60718) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for nNOS Antibody (60718) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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