nNOS Antibody (M401)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23224
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # M401
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Clone (M401) was generated from a recombinant protein that included amino acid residues within the C-terminal region of human nNOS. This human nNOS sequence has high homology with similar regions in rat and mouse nNOS. [UniProt# P29475]
Specificity
The antibody detects a 155 kDa protein on SDS-PAGE immunoblots of rat and mouse brain tissue lysates, as well in the rat pituitary cell line GC.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
155 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for nNOS Antibody (M401)
Immunocytochemistry/Immunofluorescence: nNOS Antibody (M401) [NBP3-23224] -
Immunocytochemistry/Immunofluorescence: nNOS Antibody (M401) [NBP3-23224] - Immunocytochemical labeling of nNOS phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with mouse monoclonal (mAb) nNOS (NBP3-23224), and rabbit polyclonal (pAb) nNOS (C-terminal region), nNOS (Tyr-895)/eNOS (Tyr-657), and nNOS (Tyr-1326)/iNOS (Tyr-1055). The antibodies were detected using appropriate secondary antibody conjugated to DyLight(R) 594.
Western Blot: nNOS Antibody (M401) [NBP3-23224] -
Western Blot: nNOS Antibody (M401) [NBP3-23224] - Western blot analysis of nNOS expression in adult mouse brain (lanes 1 & 3) and rat GC cells (lanes 2 & 4). The blots were probed with mouse monoclonal anti-nNOS (C-terminal region) at 1:1000 (NBP3-23224) (lanes 1 & 2) or rabbit polyclonal anti-nNOS at 1:250 (NBP3-23225) (lanes 3 & 4).Applications for nNOS Antibody (M401)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:200
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: nNOS
Long Name
Neuronal Nitric Oxide Synthase
Alternate Names
NOS1
Gene Symbol
NOS1
Additional nNOS Products
Product Documents for nNOS Antibody (M401)
Product Specific Notices for nNOS Antibody (M401)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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