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nNOS Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-858

Novus Biologicals, part of Bio-Techne
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NB100-858

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Guinea Pig

Cited:

Human, Mouse, Rat, Guinea Pig

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry Whole-Mount, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Synthetic peptide with sequence ESKKDTDEVFSS, representing the C-terminus of the human protein (residues 1423-1434) according to NP_000611.

Reactivity Notes

Guinea Pig reactivity reported in scientific literature (PMID: 26397368). Mouse reactivity reported in scientific literature (PMID: 26073142). Rat reactivity reported in scientific literature (PMID: 32278831).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

161 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for nNOS Antibody

Immunocytochemistry/ Immunofluorescence: nNOS Antibody [NB100-858]

Immunocytochemistry/ Immunofluorescence: nNOS Antibody [NB100-858]

Immunocytochemistry/Immunofluorescence: nNOS Antibody [NB100-858] - Action potentials were evoked with depolarizing current pulses. (A) Neuron from colonic specimen of non-treated patient fired 1 action potential in response to a depolarizing current. (A') Intracellular injection of carboxyfluorescein during recording confirmed Dogiel type I, uniaxonal morphology. (A") IHC demonstrated neuron was Hu-immunoreactive but did not contain nNOS immunoreactivity. (B) Neuron from a chemotherapy-treated patient fired multiple action potentials in response to a smaller depolarising current pulse. (B') Intracellular injection of carboxyfluorescein confirmed neuronal morphology. (B") This neuron was Hu-immunoreactive, but not nNOS-immunoreactive, scale bar = 100 um. Image collected & cropped by CiteAb from the following publication (www.onlinelibrary.wiley.com/doi/10.1111/nmo.12795) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: nNOS Antibody [NB100-858]

Immunohistochemistry-Paraffin: nNOS Antibody [NB100-858]

Immunohistochemistry-Paraffin: nNOS Antibody [NB100-858] - Staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Antibody at 2.5 ug/mL.
Flow Cytometry: nNOS Antibody [NB100-858]

Flow Cytometry: nNOS Antibody [NB100-858]

Flow Cytometry: nNOS Antibody [NB100-858] - Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for nNOS Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry-Paraffin

2.5 ug/mL

Peptide ELISA

detection limit 1:64000
Application Notes
Successful use in ICC/IF has been reported in the literature (PMID: 20140458). It has also been used succesfully in IHC (PMID: 20140458). Use in IHC-Frozen and IHC-whole mount reported in scientific literature (PMID 24728885). Use in Western Blot reported in scientific literature (PMID: 32278831).

Reviewed Applications

Read 1 review rated 4 using NB100-858 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: nNOS

Three isoforms of nitric oxide synthase (NOS) have been identified. All are homodimers with subunits of 130-160 kDa. All have binding sites for NADPH, FAD, and FMN near the carboxyl terminus (the reductase domain), and binding sites for tetrahydrobiopterin (BH4) and heme near the amino terminus (the oxygenase domain). The reductase and oxygenase domains are linked by a calmodulin (CaM) binding site. Occupation of this site facilitates electron transfer from the cofactors in the reductase domain to heme during nitric oxide production. NOS catalyzes the conversion of arginine to citrulline and nitric oxide (NO). Neuronal nitric oxide synthase (nNOS, bNOS, cNOS, Type I) is associated with the post-synaptic density protein (PSD-95) in the neuronal membrane. In response to increased intracellular Ca2+, nNOS interacts with CaM. The Ca2+-CaM complex, in combination with BH4, binds to nNOS and induces its translocation from the plasma membrane to the cytoplasm. The dephosphorylation of nNOS by calcineurin initiates the production NO. NO activates guanylyl cyclase (GC) and activates the various cGMP-regulated signaling pathways. nNOS is in activated by phosphorylation by protein kinase A (PKA) or protein kinase C (PKC).

Long Name

Neuronal Nitric Oxide Synthase

Alternate Names

NOS1

Entrez Gene IDs

4842 (Human); 18125 (Mouse); 24598 (Rat)

Gene Symbol

NOS1

Additional nNOS Products

Product Documents for nNOS Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for nNOS Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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