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nNOS Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-39681

Novus Biologicals, part of Bio-Techne
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NBP1-39681

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Bat, Canine, Equine, Monkey, Primate, Rabbit

Cited:

Human, Rat

Predicted:

Chicken (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Human nNOS amino acids 1422-1433 (ESKKDTDEVFSS).

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Marmoset (100%), Xenopus (83%), Zebrafish (83%).

Specificity

Human nNOS amino acids 1422-1433 (ESKKDTDEVFSS)1,2

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for nNOS Antibody

Western Blot: nNOS Antibody [NBP1-39681]

Western Blot: nNOS Antibody [NBP1-39681]

Western Blot: nNOS Antibody [NBP1-39681] - Effects of ER alpha and ER beta Antagonists on NE Regulation of VMN nNOS Protein Expression. Micropunch-dissected VMN tissue was obtained from groups of ovariectomized, estradiol-replaced female rats pretreated by intra-VMN delivery of MPP, PHTPP, or vehicle (Veh) prior to NE administration for Western blot analysis of neuronal nitric oxide synthase (nNOS) - Panel 2A,F(5, 12)=12.67,p<.0001 - protein expression. Data depict mean normalized protein optical density (O.D.) values+/-SEMfor groups of rats (n=6 per group) infused with Veh (white bars) or NE (gray bars) after delivery of Veh (solid bars), MPP (diagonal-striped bars), or PHTPP (cross-hatched bars) administration. *p<.05; **p<.01; ***p<.001. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32233668/) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: nNOS Antibody [NBP1-39681]

Immunohistochemistry-Paraffin: nNOS Antibody [NBP1-39681]

Immunohistochemistry-Paraffin: nNOS Antibody [NBP1-39681] - Analysis of anti-nNOS / NOS1 antibody with human brain, cerebellum at concentration 5 ug/ml.
Western Blot: nNOS Antibody [NBP1-39681]

Western Blot: nNOS Antibody [NBP1-39681]

Western Blot: nNOS Antibody [NBP1-39681]

Applications for nNOS Antibody

Application
Recommended Usage

Immunocytochemistry

1:10 - 1:500

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, pH 7.2, containing 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: nNOS

NOS (nitric oxide synathase) is a mediator of NO (nitric oxide) production, an inorganic gaseous messenger between cells. In cells, NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Three isoforms of NOS has been identified (eNOS, iNOS, and nNOS) with all of them having binding sites for NADPH, FAD, FMN, and tetrahydrobiopterin (1). Specifically, nNOS (Neuronal nitric oxide synthase bNOS, cNOS, Type I) is bound to PSD95 located in the neuronal membrane and at increased level of Ca2+, nNOS is trasnlocated to cytoplasm. In cytoplasm, nNOS is dephosphorylated by calcineurin which initiates NO production (2). A phosphorylation by PKA and PKC on nNOS leads down-regulation of NO production.

Long Name

Neuronal Nitric Oxide Synthase

Alternate Names

NOS1

Entrez Gene IDs

4842 (Human); 24598 (Rat)

Gene Symbol

NOS1

UniProt

Additional nNOS Products

Product Documents for nNOS Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for nNOS Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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