nNOS Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23225

Novus Biologicals, part of Bio-Techne
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NBP3-23225
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all nNOS Antibodies »

Product Summary for nNOS Antibody

Immunogen

Amino acid residues within the C-terminal region of human nNOS. This human nNOS sequence has high homology with similar regions in rat and mouse nNOS. [UniProt# P29475]

Specificity

The antibody detects a 155 kDa protein on SDS-PAGE immunoblots of rat and mouse brain tissue lysates, as well in the rat pituitary cell line GC.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

155 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for nNOS Antibody

nNOS Antibody

Western Blot: nNOS Antibody [NBP3-23225] -

Western Blot: nNOS Antibody [NBP3-23225] - Western blot analysis of nNOS expression in adult mouse brain (lanes 1 & 3) and rat GC cells (lanes 2 & 4). The blots were probed with mouse monoclonal anti-nNOS (C-terminal region) at 1:1000 (NBP3-23224) (lanes 1 & 2) or rabbit polyclonal anti-nNOS at 1:250 (NBP3-23225) (lanes 3 & 4).

Applications for nNOS Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry

1:300

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: nNOS

NOS (nitric oxide synathase) is a mediator of NO (nitric oxide) production, an inorganic gaseous messenger between cells. In cells, NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Three isoforms of NOS has been identified (eNOS, iNOS, and nNOS) with all of them having binding sites for NADPH, FAD, FMN, and tetrahydrobiopterin (1). Specifically, nNOS (Neuronal nitric oxide synthase bNOS, cNOS, Type I) is bound to PSD95 located in the neuronal membrane and at increased level of Ca2+, nNOS is trasnlocated to cytoplasm. In cytoplasm, nNOS is dephosphorylated by calcineurin which initiates NO production (2). A phosphorylation by PKA and PKC on nNOS leads down-regulation of NO production.

Long Name

Neuronal Nitric Oxide Synthase

Alternate Names

NOS1

Gene Symbol

NOS1

Additional nNOS Products

Product Documents for nNOS Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for nNOS Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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