NNT Antibody (1D6)

Novus Biologicals, part of Bio-Techne | Catalog # H00023530-M01

Novus Biologicals, part of Bio-Techne
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H00023530-M01
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Key Product Details

Species Reactivity

Human, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1D6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NNT Antibodies »

Product Specifications

Immunogen

NNT (AAH32370, 1 a.a. ~ 207 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MNRSLANVILGGYGTTSTAGGKPMEISGTHTEINLDNAIDMIREANSIIITPGYGLCAAKAQYPIADLVKMLTEQGKKVRFGIHPVAGRMPGQLNVLLAEAGVPYDIVLEMDEINHDFPDTDLVLVIGANDTVNSAAQEDPNSIIAGMPVLEVWKSKQVIVMKRSLGVGYAAVDNPIFYKPNTAMLLGDAKKTCDALQAKVRESYQK

Reactivity Notes

Human. Other species not tested.

Specificity

NNT - nicotinamide nucleotide transhydrogenase

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for NNT Antibody (1D6)

Western Blot: NNT Antibody (1D6) [H00023530-M01]

Western Blot: NNT Antibody (1D6) [H00023530-M01]

Western Blot: NNT Antibody (1D6) [H00023530-M01] - NNT monoclonal antibody (M01), clone 1D6 Analysis of NNT expression in HepG2.
Immunocytochemistry/ Immunofluorescence: NNT Antibody (1D6) [H00023530-M01]

Immunocytochemistry/ Immunofluorescence: NNT Antibody (1D6) [H00023530-M01]

Immunocytochemistry/Immunofluorescence: NNT Antibody (1D6) [H00023530-M01] - Analysis of monoclonal antibody to NNT on HepG2 cell. Antibody concentration 10 ug/ml.
ELISA: NNT Antibody (1D6) [H00023530-M01]

ELISA: NNT Antibody (1D6) [H00023530-M01]

ELISA: NNT Antibody (1D6) [H00023530-M01] - Detection limit for recombinant GST tagged NNT is approximately 0.3ng/ml as a capture antibody.
View 1 more images

Applications for NNT Antibody (1D6)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NNT

This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification. Two alternatively spliced variants, encoding the same protein, have been found for this gene.

Alternate Names

MGC126502, mitochondrial, nicotinamide nucleotide transhydrogenaseMGC126503, Pyridine nucleotide transhydrogenase

Entrez Gene IDs

23530 (Human)

Gene Symbol

NNT

OMIM

607878 (Human)

UniProt

AAH32370 (Human)

Additional NNT Products

Product Documents for NNT Antibody (1D6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NNT Antibody (1D6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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