Nogo Receptor/NgR Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1536
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-LQALPDDTFRDLGN corresponding to internal region according to NP_075380.1.
Epitope
internal region
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Nogo Receptor/NgR Antibody
![Western Blot: Nogo Receptor/NgR Antibody [NB100-1536]](https://resources.bio-techne.com/images/products/Nogo-Receptor-NgR-Antibody-Western-Blot-NB100-1536-img0002.jpg "Western Blot: Nogo Receptor/NgR Antibody [NB100-1536]")
Western Blot: Nogo Receptor/NgR Antibody [NB100-1536]
Western Blot: Nogo Receptor/NgR Antibody [NB100-1536] - (0.3ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.Applications for Nogo Receptor/NgR Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:16000
Western Blot
0.3 - 1 ug/ml
Application Notes
WB: Experiments gave bands at approx. 90-100 kDa band and 37+40+45 kDa band in human brain lysate after 0.3ug/ml antibody staining. These bands were successfully blocked by the immunizing peptide and they correspond to earlier findings in scientific literature with different antibodies (Wang et al, J Neurosci. 2002 Jul 1;22(13): 5505-15). This protein has a calculated MW of 50.7 kDa band according to NP_075380.1.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Nogo Receptor/NgR
Long Name
Nogo-66 Receptor/Reticulon 4 Receptor
Alternate Names
NgR1, NogoR, RTN4R
Entrez Gene IDs
65078 (Human)
Gene Symbol
RTN4R
UniProt
Additional Nogo Receptor/NgR Products
Product Documents for Nogo Receptor/NgR Antibody
Product Specific Notices for Nogo Receptor/NgR Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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