NOL11 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90522
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: FTLSSVVLSAGPEGLLGVEQSDKTDQFLVTDSGRTVILYKVSDQKPLGSWSVKQGQIITCPAVCNFQTGEYVVVHDNKVLRIWNNEDVNL
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 25756904). Rat (87%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NOL11 Antibody
![Immunocytochemistry/ Immunofluorescence: NOL11 Antibody [NBP1-90522]](https://resources.bio-techne.com/images/products/NOL11-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90522-img0008.jpg "Immunocytochemistry/ Immunofluorescence: NOL11 Antibody [NBP1-90522]")
Immunocytochemistry/ Immunofluorescence: NOL11 Antibody [NBP1-90522]
Immunocytochemistry/Immunofluorescence: NOL11 Antibody [NBP1-90522] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoli.![Immunohistochemistry-Paraffin: NOL11 Antibody [NBP1-90522]](https://resources.bio-techne.com/images/products/NOL11-Antibody-Immunohistochemistry-Paraffin-NBP1-90522-img0002.jpg "Immunohistochemistry-Paraffin: NOL11 Antibody [NBP1-90522]")
Immunohistochemistry-Paraffin: NOL11 Antibody [NBP1-90522]
Immunohistochemistry-Paraffin: NOL11 Antibody [NBP1-90522] - Staining of human hippocampus shows strong nucleolar positivity in neuronal cells.
Analysis in human cell line RH-30.
Analysis in human cell line RH-30.Applications for NOL11 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NOL11
Alternate Names
nucleolar protein 11
Gene Symbol
NOL11
Additional NOL11 Products
Product Documents for NOL11 Antibody
Product Specific Notices for NOL11 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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