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NOL6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19562

Novus Biologicals, part of Bio-Techne
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NBP2-19562

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human NOL6. The exact sequence is proprietary.

Localization

Nucleus; nucleolus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NOL6 Antibody

Western Blot: NOL6 Antibody [NBP2-19562]

Western Blot: NOL6 Antibody [NBP2-19562]

Western Blot: NOL6 Antibody [NBP2-19562] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 5% SDS PAGE gel, diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: NOL6 Antibody [NBP2-19562]

Immunocytochemistry/ Immunofluorescence: NOL6 Antibody [NBP2-19562]

Immunocytochemistry/Immunofluorescence: NOL6 Antibody [NBP2-19562] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NOL6 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.
Western Blot: NOL6 Antibody [NBP2-19562]

Western Blot: NOL6 Antibody [NBP2-19562]

Western Blot: NOL6 Antibody [NBP2-19562] - Sample (30 ug of whole cell lysate) A: U87-MG 12% SDS PAGE gel, diluted at 1:1000.

Applications for NOL6 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NOL6

The nucleolus is a dense subnuclear membraneless organelle that assembles around clusters of rRNA genes and functions in ribosome biogenesis. This gene encodes a nucleolar RNA-associated protein that is highly conserved between species. RNase treatment of permeabilized cells indicates that the nucleolar localization is RNA dependent. Further studies suggest that the protein is associated with ribosome biogenesis through an interaction with pre-rRNA primary transcripts. Alternative splicing has been observed at this locus and two splice variants encoding distinct isoforms have been identified. [provided by RefSeq]

Alternate Names

bA311H10.1, FLJ21959, MGC14896, MGC14921, MGC20838, Nrap, nucleolar protein 6, nucleolar protein family 6 (RNA-associated), Nucleolar RNA-associated protein, UTP22

Gene Symbol

NOL6

UniProt

Additional NOL6 Products

Product Documents for NOL6 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NOL6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.