Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all NOL8 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 1117 and 1167 of human nucleolar protein Nop132 using the numbering given in entry NP_060418.4 (GeneID 55035).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NOL8 Antibody

Western Blot: NOL8 Antibody [NBP1-46850]

Western Blot: NOL8 Antibody [NBP1-46850]

Western Blot: NOL8 Antibody [NBP1-46850] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NOP132 antibody NBP1-46850 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. NOP132 was also immunoprecipitated by rabbit anti-NOP132 antibody NBP1-46849, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Immunohistochemistry: NOL8 Antibody [NBP1-46850]

Immunohistochemistry: NOL8 Antibody [NBP1-46850]

Immunohistochemistry: NOL8 Antibody [NBP1-46850] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- NOP132 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB

Applications for NOL8 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NOL8

NOL8 binds Ras-related GTP-binding proteins (see MIM 608267) and plays a role in cell growth (Sekiguchi et al., 2004 (PubMed 14660641)).(supplied by OMIM)

Alternate Names

NOP132, nucleolar protein 8

Gene Symbol

NOL8

Additional NOL8 Products

Product Documents for NOL8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NOL8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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