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NOLC1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-22982

Novus Biologicals, part of Bio-Techne
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NBP1-22982

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

Product Summary for NOLC1 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 580 and 630 of human nucleolar and coiled-body phosphoprotein 1 using the numbering given in entry NP_004732.2 (GeneID 9221).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NOLC1 Antibody

Western Blot: NOLC1 Antibody [NBP1-22982]

Western Blot: NOLC1 Antibody [NBP1-22982]

Western Blot: NOLC1 Antibody [NBP1-22982] - Whole cell lysate from K562 (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and HeLa (H; 50 mcg) cells. Affinity purified rabbit anti- NOPP140 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. NOPP140 was also immunoprecipitated by rabbit anti-NOPP140 antibody NBP1-22981, which recognizes an upstream epitope.

Applications for NOLC1 Antibody

Application
Recommended Usage

Immunoprecipitation

5-15 ug/mg lysate

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NOLC1

NOPP140 (140 kDa nucleolar phosphoprotein) is implicated to play a role in ribosome assembly and the regulation of rRNA transcription. It has been observed to shuttle between the nucleus and cytoplasm and may also be important to nucleolar structural organization.

Alternate Names

140 kDa nucleolar phosphoprotein, HCV NS5A trans-regulated protein 13, HCV NS5A-transactivated protein 13, Hepatitis C virus NS5A-transactivated protein 13, KIAA0035NS5ATP13, NOPP130, Nopp140, Nucleolar 130 kDa protein, nucleolar and coiled-body phosphoprotein 1, nucleolar and coiled-body phosphprotein 1, Nucleolar phosphoprotein p130, nucleolar protein p130, P130

Entrez Gene IDs

9221 (Human)

Gene Symbol

NOLC1

UniProt

Additional NOLC1 Products

Product Documents for NOLC1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NOLC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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