non-muscle Myosin IIA Antibody (4H3)
Novus Biologicals, part of Bio-Techne | Catalog # H00004627-M06
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 4H3
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
MYH9 (NP_002464.1, 1871 a.a. ~ 1960 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RLKQLKRQLEEAEEEAQRANASRRKLQRELEDATETADAMNREVSSLKNKLRRGDLPFVVPRRMARKGAGDGSDEEVDGKADGAEAKPAE
Specificity
myosin, heavy polypeptide 9, non-muscle
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for non-muscle Myosin IIA Antibody (4H3)
Immunocytochemistry/ Immunofluorescence: non-muscle Myosin IIA Antibody (4H3) [H00004627-M06]
Immunocytochemistry/Immunofluorescence: non-muscle Myosin IIA Antibody (4H3) [H00004627-M06] - Analysis of monoclonal antibody to MYH9 on HeLa cell. Antibody concentration 10 ug/mlImmunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody (4H3) [H00004627-M06]
Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody (4H3) [H00004627-M06] - Analysis of monoclonal antibody to MYH9 on formalin-fixed paraffin-embedded human ovarian cancer. Antibody concentration 0.7 ug/mlELISA: non-muscle Myosin IIA Antibody (4H3) [H00004627-M06]
ELISA: non-muscle Myosin IIA Antibody (4H3) [H00004627-M06] - Detection limit for recombinant GST tagged MYH9 is approximately 0.03ng/ml as a capture antibody.Applications for non-muscle Myosin IIA Antibody (4H3)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Non-muscle Myosin IIA
Long Name
Myosin-9
Alternate Names
MYH9, NMMHC II-a, NMMHC-A, NMMHC-IIA
Entrez Gene IDs
4627 (Human)
Gene Symbol
MYH9
OMIM
153640 (Human)
Additional Non-muscle Myosin IIA Products
Product Documents for non-muscle Myosin IIA Antibody (4H3)
Product Specific Notices for non-muscle Myosin IIA Antibody (4H3)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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