non-muscle Myosin IIA Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23426
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for non-muscle Myosin IIA Antibody
Immunogen
non-muscle Myosin IIA synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human non-muscle heavy chain. This peptide sequence is highly conserved in rat and mouse non-muscle Myosin IIA, but is not found in non-muscle Myosin IIB and is not well conserved in non-muscle Myosin IIC. [UniProt# P35579]
Specificity
The antibody detects a 200 kDa protein corresponding to the apparent molecular mass of non-muscle Myosin IIA on SDS-PAGE immunoblots of human A431, Jurkat, mouse C2C12, and rat PC12 cells.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
200 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for non-muscle Myosin IIA Antibody
Immunocytochemistry/Immunofluorescence: non-muscle Myosin IIA Antibody [NBP3-23426] -
Immunocytochemistry/Immunofluorescence: non-muscle Myosin IIA Antibody [NBP3-23426] - Immunocytochemical labeling of Slingshot-1L in rat PC12 cells differentiated with NGF. The cells were labeled with rabbit polyclonal anti-Myosin IIA Heavy Chain (a.a. 1936-1950), then detected using appropriate secondary antibody conjugated to Cy3. The antibody was used in the absence (left) or presence (right) of blocking peptide.
Western Blot: non-muscle Myosin IIA Antibody [NBP3-23426] -
Western Blot: non-muscle Myosin IIA Antibody [NBP3-23426] - Western blot image of human A431 cells. The blots were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4), then probed with rabbit polyclonal Myosin IIA Heavy Chain (Ser-1943), phospho-specific antibody (lanes 1 & 2) or rabbit polyclonal Myosin IIA Heavy Chain (a.a. 1936-1950) antibody (lanes 3 & 4).Applications for non-muscle Myosin IIA Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Non-muscle Myosin IIA
Long Name
Myosin-9
Alternate Names
MYH9, NMMHC II-a, NMMHC-A, NMMHC-IIA
Gene Symbol
MYH9
Additional Non-muscle Myosin IIA Products
Product Documents for non-muscle Myosin IIA Antibody
Product Specific Notices for non-muscle Myosin IIA Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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