non-muscle Myosin IIA [p Ser1803] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23430
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Chicken
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for non-muscle Myosin IIA [p Ser1803] Antibody
Immunogen
Phospho-non-muscle Myosin IIA (Ser-1803) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Ser-1803 in human myosin IIA heavy chain. This peptide sequence is highly conserved in rat and mouse myosin IIA heavy chain, and the site has some homology to the conserved sites in non-muscle myosin IIB and smooth muscle myosin heavy chains. [UniProt# P35579]
Modification
p Ser1803
Specificity
This antibody was cross-adsorbed to unphosphorylated non-muscle Myosin IIA (Ser-1803) peptide before affinity purification using phospho-non-muscle Myosin IIA (Ser-1803) peptide (without carrier). The antibody detects a 200 kDa protein corresponding to non-muscle Myosin IIA on SDS-PAGE immunoblots of human A431 cells treated with Calyculin A. This reactivity is removed after treatment with lambda phosphatase.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
200 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for non-muscle Myosin IIA [p Ser1803] Antibody
![non-muscle Myosin IIA [p Ser1803] Antibody](https://resources.bio-techne.com/images/products/nbp3-23430_rabbit-non-muscle-myosin-iia-p-ser1803-pab-189202317355218.jpg "Western Blot: non-muscle Myosin IIA [p Ser1803] Antibody [NBP3-23430] -")
Western Blot: non-muscle Myosin IIA [p Ser1803] Antibody [NBP3-23430] -
Western Blot: non-muscle Myosin IIA [p Ser1803] Antibody [NBP3-23430] - Western blot image of human A431 cells stimulated with calyculin A (100 nM, 30 min). The blots were untreated (lanes 1, 3 & 5) or treated with lambda phosphatase (lanes 2, 4 & 6), and probed with rabbit polyclonals Myosin IIA Heavy Chain (a.a. 1936-1950) (lanes 1 & 2), Myosin IIA Heavy Chain (Ser-1803), phospho-specific (lanes 3 & 4) or Myosin IIA Heavy Chain (Ser-1916) phospho-specific (lanes 5 & 6).Applications for non-muscle Myosin IIA [p Ser1803] Antibody
Application
Recommended Usage
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Non-muscle Myosin IIA
Long Name
Myosin-9
Alternate Names
MYH9, NMMHC II-a, NMMHC-A, NMMHC-IIA
Gene Symbol
MYH9
Additional Non-muscle Myosin IIA Products
Product Documents for non-muscle Myosin IIA [p Ser1803] Antibody
Product Specific Notices for non-muscle Myosin IIA [p Ser1803] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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