non-muscle Myosin IIA [p Ser1943] Antibody

Biological Validation

Validated:

Unconjugated

Polyclonal Rabbit IgG

Please see the vial label for concentration. If unlisted please contact technical services.

Phospho-non-muscle Myosin IIA (Ser-1943) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Ser-1943 in human myosin IIA heavy chain. This peptide sequence is highly conserved in rat and mouse non-muscle Myosin IIA, but is not found in non-muscle Myosin IIB and is not well conserved in non-muscle Myosin IIC. [UniProt# P35579]

p Ser1943

This antibody was cross-adsorbed to unphosphorylated NMHC-IIA (Ser-1943) peptide before affinity purification using phospho-NMHC-IIA (Ser-1943) peptide (without carrier). The antibody detects a 200 kDa* protein corresponding to NMHC-IIA on SDS-PAGE immunoblots of human A431, Jurkat, mouse C2C12, and rat PC12 cells. This reactivity is removed after treatment with lambda phosphatase. The antibody also detects NMHC-IIA (Ser-1943) phosphorylation in chick Fibroblasts.

Polyclonal

Rabbit

IgG

200 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Stable for at least 1 year at -20°C.

Antigen Affinity-purified

PBS, 1 mg/ml BSA and 50% glycerol

0.05% Sodium Azide

Please see the vial label for concentration. If unlisted please contact technical services.

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at -20C.