NONO Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-40789

Novus Biologicals, part of Bio-Techne
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NB100-40789
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all NONO Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-NRAAPGAEFAPNK corresponding to C-Terminus according to NP_031389.3.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for NONO Antibody

Western Blot: NONO Antibody [NB100-40789]

Western Blot: NONO Antibody [NB100-40789]

Western Blot: NONO Antibody [NB100-40789] - Analysis of NONO using this antibody at 0.1 ug/ml in Mouse Brain lysate (35ug protein in RIPA buffer).
Immunohistochemistry-Paraffin: NONO Antibody [NB100-40789]

Immunohistochemistry-Paraffin: NONO Antibody [NB100-40789]

Immunohistochemistry-Paraffin: NONO Antibody [NB100-40789] - (3.75ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: NONO Antibody [NB100-40789]

Immunohistochemistry-Paraffin: NONO Antibody [NB100-40789]

Immunohistochemistry-Paraffin: NONO Antibody [NB100-40789] - (3.8ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for NONO Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 6 ug/ml

Immunohistochemistry-Paraffin

1:200

Peptide ELISA

Detection limit 1:16000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 60 kDa band observed in mouse brain lysates (calculated MW of 54.2 kDa band according to human NP_031389.3 and 54.5 kDa band according to mouse NP_075633.2). In transfected HEK293 transiently expressing human NONO a band of approx. 55 kDa is observed. This band is not observed in the non-transfected HEK293. The endogenous 50 kDa band protein is observed in both. IHC-P: Human spleen shows nuclear staining in select splenocytes.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NONO

This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined.

Alternate Names

NMT5552 kDa subunit, non-POU domain containing, octamer-binding, NRB54non-POU-domain-containing, octamer-binding, p54(nrb)

Entrez Gene IDs

4841 (Human); 53610 (Mouse); 317259 (Rat)

Gene Symbol

NONO

UniProt

NP_031389.3 (Human)

Additional NONO Products

Product Documents for NONO Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NONO Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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