NOP56 Antibody (CL2603)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36778

Novus Biologicals, part of Bio-Techne
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NBP2-36778
NBP2-36778-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2603

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NOP56 Antibodies »

Product Summary for NOP56 Antibody (CL2603)

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids:EDPSISFSKPKKKKSFSKEELMSSDLEETAGSTS

Epitope

FSKEELMSSDLEETA

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for NOP56 Antibody (CL2603)

Western Blot: NOP56 Antibody (CL2603) [NBP2-36778]

Western Blot: NOP56 Antibody (CL2603) [NBP2-36778]

Western Blot: NOP56 Antibody (CL2603) [NBP2-36778] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-NOP56 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: NOP56 Antibody (CL2603) [NBP2-36778]

Immunocytochemistry/ Immunofluorescence: NOP56 Antibody (CL2603) [NBP2-36778]

Immunocytochemistry/Immunofluorescence: NOP56 Antibody (CL2603) [NBP2-36778] - Staining in MCF7 cell line with Anti-NOP56 monoclonal antibody, showing nucleoli-specific staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778]

Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778]

Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778] - Staining of human breast cancer shows strong nucleolar positivity in tumor cells.
View 10 more images

Applications for NOP56 Antibody (CL2603)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NOP56

Nop56p is a yeast nucleolar protein that is part of a complex with the nucleolar proteins Nop58p and fibrillarin. Nop56p is required for assembly of the 60S ribosomal subunit and is involved in pre-rRNA processing. The protein encoded by this gene is similar in sequence to Nop56p and is also found in the nucleolus. Multiple transcript variants encoding several different isoforms have been found for this gene, but the full-length nature of most of them have not been determined.

Alternate Names

NOL5A, NOP56 ribonucleoprotein homolog (yeast), nucleolar protein 56, Nucleolar protein 5A, nucleolar protein 5A (56kD with KKE/D repeat), nucleolar protein 5A (56kDa with KKE/D repeat)

Gene Symbol

NOP56

UniProt

O00567 (Human)

Additional NOP56 Products

Product Documents for NOP56 Antibody (CL2603)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NOP56 Antibody (CL2603)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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