NOP56 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46847
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 494 and 544 of human NOP56 ribonucleoprotein homolog using the numbering given in entry NP_006383.2 (GeneID 10528).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NOP56 Antibody
![Western Blot: NOP56 Antibody [NBP1-46847]](https://resources.bio-techne.com/images/products/NOP56-Antibody-Western-Blot-NBP1-46847-img0002.jpg "Western Blot: NOP56 Antibody [NBP1-46847]")
Western Blot: NOP56 Antibody [NBP1-46847]
Western Blot: NOP56 Antibody [NBP1-46847] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NOP56 antibody NBP1-46847 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. NOP56 was also immunoprecipitated by rabbit anti-NOP56 antibody NBP1-46848, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).![Immunohistochemistry-Paraffin: NOP56 Antibody [NBP1-46847]](https://resources.bio-techne.com/images/products/NOP56-Antibody-Immunohistochemistry-Paraffin-NBP1-46847-img0006.jpg "Immunohistochemistry-Paraffin: NOP56 Antibody [NBP1-46847]")
Immunohistochemistry-Paraffin: NOP56 Antibody [NBP1-46847]
Immunohistochemistry-Paraffin: NOP56 Antibody [NBP1-46847] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-NOP56 used at a dilution of 1:200 (1ug/ml). Detection: DABApplications for NOP56 Antibody
Application
Recommended Usage
Immunohistochemistry
1:100 - 1:500
Immunohistochemistry-Paraffin
1:100 - 1:500
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: NOP56
Alternate Names
NOL5A, NOP56 ribonucleoprotein homolog (yeast), nucleolar protein 56, Nucleolar protein 5A, nucleolar protein 5A (56kD with KKE/D repeat), nucleolar protein 5A (56kDa with KKE/D repeat)
Entrez Gene IDs
10528 (Human)
Gene Symbol
NOP56
UniProt
Additional NOP56 Products
Product Documents for NOP56 Antibody
Product Specific Notices for NOP56 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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