NOP56 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46847

Novus Biologicals, part of Bio-Techne
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NBP1-46847
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all NOP56 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 494 and 544 of human NOP56 ribonucleoprotein homolog using the numbering given in entry NP_006383.2 (GeneID 10528).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NOP56 Antibody

Western Blot: NOP56 Antibody [NBP1-46847]

Western Blot: NOP56 Antibody [NBP1-46847]

Western Blot: NOP56 Antibody [NBP1-46847] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NOP56 antibody NBP1-46847 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. NOP56 was also immunoprecipitated by rabbit anti-NOP56 antibody NBP1-46848, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Immunohistochemistry-Paraffin: NOP56 Antibody [NBP1-46847]

Immunohistochemistry-Paraffin: NOP56 Antibody [NBP1-46847]

Immunohistochemistry-Paraffin: NOP56 Antibody [NBP1-46847] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-NOP56 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for NOP56 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NOP56

Nop56p is a yeast nucleolar protein that is part of a complex with the nucleolar proteins Nop58p and fibrillarin. Nop56p is required for assembly of the 60S ribosomal subunit and is involved in pre-rRNA processing. The protein encoded by this gene is similar in sequence to Nop56p and is also found in the nucleolus. Multiple transcript variants encoding several different isoforms have been found for this gene, but the full-length nature of most of them have not been determined.

Alternate Names

NOL5A, NOP56 ribonucleoprotein homolog (yeast), nucleolar protein 56, Nucleolar protein 5A, nucleolar protein 5A (56kD with KKE/D repeat), nucleolar protein 5A (56kDa with KKE/D repeat)

Entrez Gene IDs

10528 (Human)

Gene Symbol

NOP56

UniProt

O00567 (Human)

Additional NOP56 Products

Product Documents for NOP56 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NOP56 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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