NOP58 Antibody (34B12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-05225

Novus Biologicals, part of Bio-Techne
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NBP1-05225-0.125ml
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Key Product Details

Species Reactivity

Yeast

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # 34B12

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all NOP58 Antibodies »

Product Specifications

Immunogen

A yeast nucleolar preparation

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NOP58 Antibody (34B12)

Western Blot: NOP58 Antibody (34B12) [NBP1-05225]

Western Blot: NOP58 Antibody (34B12) [NBP1-05225]

Western Blot: NOP58 Antibody [NBP1-05225] - Western Blot analysis of whole yeast protein extracts detect NOP58. Molecular weight detected is 57 kDa.

Applications for NOP58 Antibody (34B12)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000-1:10000

Immunoprecipitation

2-4 ul

Western Blot

1:10000
Application Notes
This NOP58 (34B12) antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In WB a band can be seen at 57 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Tissue culture supernatant

Formulation

Supplied as concentrated hybridoma cell culture media.

Preservative

0.035% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NOP58

NOP58 (nucleolar protein 58) is a member of the conserved nop5 (Drosophila)/sik1/Nop56p (yeast) gene family that encodes components of snoRNP/small nucleolar ribonucleoproteins, which contains a snoRNA and a complex of proteins. NOP58 belongs to box C/D snoRNP, which is named for short, conserved box C (UGAUGA) and box D (CUGA) sequences present in their RNA moiety. snoRNP primarily catalyzes the cleavage as well as modification of rRNA during the biogenesis of ribosomes, nucleolar sizing , and snoRNA trafficking . In yeast, at least two members, Nop56 and Nop58, are present, and yeast cells deficient in either of the two members undergoes growth arrest because of defective protein translation. Furthermore, functional mutations of Nop56p/sik1 have been shown to cause reduced cell growth and proliferation. In mammalian cells, NOP58 has been found to increase rRNA synthesis and translational efficiency of the exogenous luciferase (Luc) gene, and its expression correlate with metastatic phenotypes of clinical melanomas.

Alternate Names

HSPC120, NOL5, NOP5/NOP58, NOP58 ribonucleoprotein homolog (yeast), NOP5nucleolar protein 58, Nucleolar protein 5, nucleolar protein NOP5/NOP58

Gene Symbol

NOP58

Additional NOP58 Products

Product Documents for NOP58 Antibody (34B12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NOP58 Antibody (34B12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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