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NOSIP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85661

Novus Biologicals, part of Bio-Techne
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NBP1-85661
NBP1-85661-25ul

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RHGKNCTAGAVYTYHEKKKDTAASGYGTQNIRLSRDAVKDFDCCCLSLQPCHDPVVTPDGYLYEREAILEYILHQKKEIARQMKAYEKQRGT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NOSIP Antibody

Western Blot: NOSIP Antibody [NBP1-85661]

Western Blot: NOSIP Antibody [NBP1-85661]

Western Blot: NOSIP Antibody [NBP1-85661] - Analysis using Anti-NOSIP antibody NBP1-85661 (A) shows similar pattern to independent antibody NBP2-55693 (B).
Immunocytochemistry/ Immunofluorescence: NOSIP Antibody [NBP1-85661]

Immunocytochemistry/ Immunofluorescence: NOSIP Antibody [NBP1-85661]

Immunocytochemistry/Immunofluorescence: NOSIP Antibody [NBP1-85661] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: NOSIP Antibody [NBP1-85661]

Immunohistochemistry-Paraffin: NOSIP Antibody [NBP1-85661]

Immunohistochemistry-Paraffin: NOSIP Antibody [NBP1-85661] - Staining of human esophagus shows moderate cytoplasmic and nuclear positivity in squamous epithelial cells.

Applications for NOSIP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NOSIP

Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity

Alternate Names

CGI-25, eNOS interacting protein, eNOS-interacting protein, nitric oxide synthase interacting protein, nitric oxide synthase-interacting protein

Gene Symbol

NOSIP

Additional NOSIP Products

Product Documents for NOSIP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NOSIP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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