Notch-3 Antibody (2E4D11) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52521
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
CyTOF-ready, ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 2E4D11
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Purified recombinant fragment of human Notch-3 (AA: 47-156) expressed in E. Coli.
Reactivity Notes
Use in Mouse reported in scientific literature (PMID:32169072).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
243.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Notch-3 Antibody (2E4D11) - BSA Free
![Western Blot: Notch-3 Antibody (2E4D11)BSA Free [NBP2-52521]](https://resources.bio-techne.com/images/products/Notch-3-Antibody-2E4D11-Western-Blot-NBP2-52521-img0001.jpg "Western Blot: Notch-3 Antibody (2E4D11)BSA Free [NBP2-52521]")
Western Blot: Notch-3 Antibody (2E4D11)BSA Free [NBP2-52521]
Western Blot: Notch-3 Antibody (2E4D11) [NBP2-52521] - Analysis using Notch3 mAb against human Notch3 (AA: 47-156) recombinant protein. (Expected MW is 37.3 kDa).![Western Blot: Notch-3 Antibody (2E4D11)BSA Free [NBP2-52521]](https://resources.bio-techne.com/images/products/Notch-3-Antibody-2E4D11-Western-Blot-NBP2-52521-img0002.jpg "Western Blot: Notch-3 Antibody (2E4D11)BSA Free [NBP2-52521]")
Western Blot: Notch-3 Antibody (2E4D11)BSA Free [NBP2-52521]
Western Blot: Notch-3 Antibody (2E4D11) [NBP2-52521] - Analysis using Notch3 mAb against HEK293 (1) and Notch3 (AA: 47-156)-hIgGFc transfected HEK293 (2) cell lysate.![ELISA: Notch-3 Antibody (2E4D11) - BSA Free [NBP2-52521]](https://resources.bio-techne.com/images/products/Notch-3-Antibody-2E4D11-ELISA-NBP2-52521-img0003.jpg "ELISA: Notch-3 Antibody (2E4D11) - BSA Free [NBP2-52521]")
ELISA: Notch-3 Antibody (2E4D11) - BSA Free [NBP2-52521]
ELISA: Notch-3 Antibody (2E4D11) [NBP2-52521] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).Applications for Notch-3 Antibody (2E4D11) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Western Blot
1:500-1:2000
Application Notes
This antibody is Cytof ready. Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:32169072).
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Notch-3
Alternate Names
Notch3
Gene Symbol
NOTCH3
Additional Notch-3 Products
Product Documents for Notch-3 Antibody (2E4D11) - BSA Free
Product Specific Notices for Notch-3 Antibody (2E4D11) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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