Nox4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99108
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Nox4 Antibody
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Nox4.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Nox4 Antibody
![Immunoprecipitation: Nox4 Antibody [NBP2-99108]](https://resources.bio-techne.com/images/products/Nox4-Antibody-Immunoprecipitation-NBP2-99108-img0001.jpg "Immunoprecipitation: Nox4 Antibody [NBP2-99108]")
Immunoprecipitation: Nox4 Antibody [NBP2-99108]
Immunoprecipitation: Nox4 Antibody [NBP2-99108] - Lane A:0.5 mg 293T Whole Cell Lysate 2 uL anti-NOX4 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-NOX4 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions. Predicted band size: 64 kDaObserved band size: 71 kDaApplications for Nox4 Antibody
Application
Recommended Usage
Immunoprecipitation
1-4 ug/mg of lysate
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Nox4
NOX-4 is also expressed in fetal tissues, placenta, glioblastoma and vascular cells. Like gp91-phox, the enzymatic activity of NOX4 produces superoxide anions. In vascular cells, the addition of angiotensin II increases NOX4 expression, which indicates that NOX4 has a role in vascular oxidative stress response. NOX4 has a role as a redox messenger in the activation of intracellular signaling pathways leading (or contributing) to mitochondriogenesis, cell survival, and differentiation in hematopoietic stem cells. Data suggest that NOX4 provides a novel link between the insulin receptor and the generation of cellular reactive oxygen species that enhances insulin signal transduction.
Long Name
NADPH Oxidase 4
Alternate Names
KOX-1, RENOX
Gene Symbol
NOX4
Additional Nox4 Products
Product Documents for Nox4 Antibody
Product Specific Notices for Nox4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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