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NPEPL1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13668

Novus Biologicals, part of Bio-Techne
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NBP2-13668

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: RAFPLFTHRSGASRRLEKKTVTVEFFLVGQDNGPVEVSTLQCLANATDGVRLAARIVDTPCNEMNTDTFLEEINKVGKELGIIPTIIRDEELKTRGFG

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NPEPL1 Antibody

Western Blot: NPEPL1 Antibody [NBP2-13668]

Western Blot: NPEPL1 Antibody [NBP2-13668]

Western Blot: NPEPL1 Antibody [NBP2-13668] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-391
Immunocytochemistry/ Immunofluorescence: NPEPL1 Antibody [NBP2-13668]

Immunocytochemistry/ Immunofluorescence: NPEPL1 Antibody [NBP2-13668]

Immunocytochemistry/Immunofluorescence: NPEPL1 Antibody [NBP2-13668] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: NPEPL1 Antibody [NBP2-13668]

Immunohistochemistry-Paraffin: NPEPL1 Antibody [NBP2-13668]

Immunohistochemistry-Paraffin: NPEPL1 Antibody [NBP2-13668] - Staining of human kidney shows strong nuclear positivity in cells in tubules.

Applications for NPEPL1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NPEPL1

Probably catalyzes the removal of unsubstituted N-terminal amino acids from various peptides

Alternate Names

aminopeptidase-like 1EC 3.4.11.-, bA261P9.2, EC 3.4.11, EC 3.4.11.1, FLJ11583, FLJ42065, KIAA1974, probable aminopeptidase NPEPL1, R

Gene Symbol

NPEPL1

Additional NPEPL1 Products

Product Documents for NPEPL1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NPEPL1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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