NPM1 Antibody [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NB110-61646F

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NPM1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a C-terminal region of human mutant Nucleophosmin (within residues 250-294). [Accession# AAW67752]

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30214626).

Localization

Nucleophosmin typically resides in the nucleus. However, in patients with acute myelogenous leukemia (AML) it mutates and localizes to the cytoplasm.

Specificity

This antibody is specific for the mutant form of Nucleophosmin.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for NPM1 Antibody [FITC]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: NPM1

Nucleophosmin (NPM; also known as B23, numatrin or NO38) is a highly conserved phosphoprotein that belongs to nucleoplasmin/nucleophosmin family of nuclear chaperones. Expressed ubiquitously with nucleolar localization, NPM shuttles between nucleoli and cytoplasm during cell cycle progression. It binds ribosome to drive ribosome nuclear export and has been associated with nucleolar ribonucleoprotein structures. Chaperonin are the proteins that provide favourable conditions for the correct folding of other proteins for preventing aggregation; and NPM has the ability to acts as chaperonin for the core histones H3, H2B and H4 proteins. NPM stimulates APEX1 endonuclease activity on A/P double-stranded DNA while inhibit the same on A/P single-stranded RNA, and regulate APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA as well as the removal of oxidized rRNA molecules. BRCA2 helps NPM in regulating centrosome duplication, whereas, PLK2 mediated phosphorylation controls the centriole duplication. NPM is implicated in diverse cellular processes including cell proliferation, and regulation of tumor suppressors such as p53/TP53 and ARF.

Long Name

Nucleophosmin/Nucleolar Phosphoprotein B23

Alternate Names

B23, Nucleophosmin, Numatrin

Gene Symbol

NPM1

Additional NPM1 Products

Product Documents for NPM1 Antibody [FITC]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NPM1 Antibody [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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