NQO-1 Antibody (13A1-R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32659
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # 13A1-R
Concentration
1 mg/ml
Product Summary for NQO-1 Antibody (13A1-R)
Immunogen
Recombinant protein within Human NQO-1 aa 1-274 / 274. (Uniprot: P15559)
Localization
Cytoplasm.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NQO-1 Antibody (13A1-R)
Western Blot: NQO-1 Antibody (13A1-R) [NBP3-32659]
Western Blot: NQO-1 Antibody (13A1-R) [NBP3-32659] - Western blot analysis of NQO-1 on different lysates with Mouse anti-NQO-1 antibody (NBP3-32659) at 1/2,000 dilution.Lane 1: A549 cell lysate
Lane 2: SiHa cell lysate
Lane 3: HepG2 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: HeLa cell lysate
Lane 6: Saos-2 cell lysate (low expression)
Lysates/proteins at 30 ug/Lane.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32659) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for NQO-1 Antibody (13A1-R)
Application
Recommended Usage
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NQO-1
Long Name
NAD(P)H Dehydrogenase, Quinone 1
Alternate Names
Azoreductase, DHQU, DIA4, Diaphorase-4, Dioxin-inducible 1, DTD, Menadione Reductase, NMOR1, NQO1, QR1, Quinone Reductase 1
Gene Symbol
NQO1
Additional NQO-1 Products
Product Documents for NQO-1 Antibody (13A1-R)
Product Specific Notices for NQO-1 Antibody (13A1-R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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